2eb6

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{{Seed}}
 
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[[Image:2eb6.png|left|200px]]
 
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==Crystal structure of HpcG complexed with Mg ion==
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The line below this paragraph, containing "STRUCTURE_2eb6", creates the "Structure Box" on the page.
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<StructureSection load='2eb6' size='340' side='right'caption='[[2eb6]], [[Resolution|resolution]] 1.69&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2eb6]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EB6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EB6 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.69&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_2eb6| PDB=2eb6 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2eb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eb6 OCA], [https://pdbe.org/2eb6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2eb6 RCSB], [https://www.ebi.ac.uk/pdbsum/2eb6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2eb6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q46982_ECOLX Q46982_ECOLX]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eb/2eb6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2eb6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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HpcG catalyses the hydration of a carbon-carbon double bond without the aid of any cofactor other than a simple divalent metal ion such as Mg(2+). Since the substrate has a nearby carbonyl group, it is believed that it first isomerises to form a pair of conjugated double bonds in the enol tautomer before Michael addition of water. Previous chemical studies of the reaction, and that of the related enzyme MhpD, have failed to provide a clear picture of the mechanism. The substrate itself is unstable, preventing co-crystallisation or soaking of crystals, but oxalate is a strong competitive inhibitor. We have solved the crystal structure of the protein in the apo form, and with magnesium and oxalate bound. Modelling substrate into the active site suggests the attacking water molecule is not part of the metal coordination shell, in contrast to a previous proposal. Our model suggests that geometrically strained cis isomer intermediates do not lie on the reaction pathway, and that separate groups are involved in the isomerisation and hydration steps.
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===Crystal structure of HpcG complexed with Mg ion===
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Structure and mechanism of HpcG, a hydratase in the homoprotocatechuate degradation pathway of Escherichia coli.,Izumi A, Rea D, Adachi T, Unzai S, Park SY, Roper DI, Tame JR J Mol Biol. 2007 Jul 27;370(5):899-911. Epub 2007 May 10. PMID:17559873<ref>PMID:17559873</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17559873}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2eb6" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17559873 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17559873}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2EB6 is a 5 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EB6 OCA].
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==Reference==
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<ref group="xtra">PMID:17559873</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Adachi, T.]]
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[[Category: Large Structures]]
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[[Category: Izumi, A.]]
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[[Category: Adachi T]]
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[[Category: Park, S Y.]]
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[[Category: Izumi A]]
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[[Category: Rea, D.]]
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[[Category: Park SY]]
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[[Category: Roper, D I.]]
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[[Category: Rea D]]
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[[Category: Tame, J R.H.]]
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[[Category: Roper DI]]
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[[Category: Unzai, S.]]
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[[Category: Tame JRH]]
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[[Category: Hydratase]]
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[[Category: Unzai S]]
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[[Category: Lyase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 05:26:09 2009''
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Current revision

Crystal structure of HpcG complexed with Mg ion

PDB ID 2eb6

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