1qv0

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{{Seed}}
 
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[[Image:1qv0.png|left|200px]]
 
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==Atomic resolution structure of obelin from Obelia longissima==
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The line below this paragraph, containing "STRUCTURE_1qv0", creates the "Structure Box" on the page.
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<StructureSection load='1qv0' size='340' side='right'caption='[[1qv0]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1qv0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Obelia_longissima Obelia longissima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QV0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QV0 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=CZH:C2-HYDROPEROXY-COELENTERAZINE'>CZH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
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{{STRUCTURE_1qv0| PDB=1qv0 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qv0 OCA], [https://pdbe.org/1qv0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qv0 RCSB], [https://www.ebi.ac.uk/pdbsum/1qv0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qv0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/OBL_OBELO OBL_OBELO] Ca(2+)-dependent bioluminescence photoprotein. Displays an emission peak at 470 nm (blue light). Trace amounts of calcium ion trigger the intramolecular oxidation of the chromophore, coelenterazine into coelenteramide and CO(2) with the concomitant emission of light.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qv/1qv0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qv0 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The spatial structure of the Ca(2+)-regulated photoprotein obelin has been solved to resolution of 1.1A. Two oxygen atoms are revealed substituted at the C2-position of the coelenterazine in contrast to the obelin structure at 1.73A resolution where one oxygen atom only was disclosed. The electron density of the second oxygen atom was very weak but after exposing the crystals to a trace of Ca(2+), the electron densities of both oxygen atoms became equally intense. In addition, one Ca(2+) was found bound in the loop of the first EF-hand motif. Four of the ligands were provided by protein residues Asp30, Asn32, Asn34, and the main chain oxygen of Lys36. The other two were from water molecules. From a comparison of B-factors for the residues constituting the active site, it is suggested that the variable electron densities observed in various photoprotein structures could be attributed to different mobilities of the peroxy oxygen atoms.
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===Atomic resolution structure of obelin from Obelia longissima===
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Atomic resolution structure of obelin: soaking with calcium enhances electron density of the second oxygen atom substituted at the C2-position of coelenterazine.,Liu ZJ, Vysotski ES, Deng L, Lee J, Rose J, Wang BC Biochem Biophys Res Commun. 2003 Nov 14;311(2):433-9. PMID:14592432<ref>PMID:14592432</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_14592432}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1qv0" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 14592432 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_14592432}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1QV0 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Obelia_longissima Obelia longissima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QV0 OCA].
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==Reference==
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<ref group="xtra">PMID:14592432</ref><references group="xtra"/>
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[[Category: Obelia longissima]]
[[Category: Obelia longissima]]
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[[Category: Renilla-luciferin 2-monooxygenase]]
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[[Category: Deng L]]
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[[Category: Deng, L.]]
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[[Category: Lee J]]
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[[Category: Lee, J.]]
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[[Category: Liu ZJ]]
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[[Category: Liu, Z J.]]
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[[Category: Rose J]]
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[[Category: Rose, J.]]
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[[Category: Vysotski ES]]
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[[Category: Vysotski, E S.]]
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[[Category: Wang BC]]
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[[Category: Wang, B C.]]
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[[Category: Atomic resolution]]
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[[Category: Bioluminescence]]
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[[Category: Calcium binding]]
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[[Category: Crystal structure]]
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[[Category: Ef-hand]]
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[[Category: Obelin]]
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[[Category: Photoprotein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 05:44:25 2009''
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Current revision

Atomic resolution structure of obelin from Obelia longissima

PDB ID 1qv0

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