2q0o

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{{Seed}}
 
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[[Image:2q0o.png|left|200px]]
 
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==Crystal structure of an anti-activation complex in bacterial quorum sensing==
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The line below this paragraph, containing "STRUCTURE_2q0o", creates the "Structure Box" on the page.
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<StructureSection load='2q0o' size='340' side='right'caption='[[2q0o]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2q0o]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sinorhizobium_fredii_NGR234 Sinorhizobium fredii NGR234]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q0O FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LAE:3-OXO-OCTANOIC+ACID+(2-OXO-TETRAHYDRO-FURAN-3-YL)-AMIDE'>LAE</scene></td></tr>
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{{STRUCTURE_2q0o| PDB=2q0o | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q0o OCA], [https://pdbe.org/2q0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q0o RCSB], [https://www.ebi.ac.uk/pdbsum/2q0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q0o ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRAR_SINFN TRAR_SINFN] Positive regulation of conjugal transfer. TraR activates target genes in the presence of AAI and also activates traR and traI themselves (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q0/2q0o_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2q0o ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bacteria can communicate via diffusible signal molecules they generate and release to coordinate their behavior in response to the environment. Signal molecule concentration is often proportional to bacterial population density, and when this reaches a critical concentration, reflecting a bacterial quorum, specific behaviors including virulence, symbiosis, and horizontal gene transfer are activated. Quorum-sensing regulation in many Gram-negative bacteria involves acylated homoserine lactone signals that are perceived through binding to LuxR-type, acylated-homoserine-lactone-responsive transcription factors. Bacteria of the rhizobial group employ the LuxR-type transcriptional activator TraR in quorum sensing, and its activity is further regulated through interactions with the TraM antiactivator. In this study, we have crystallographically determined the 3D structure of the TraR-TraM antiactivation complex from Rhizobium sp. strain NGR234. Unexpectedly, the antiactivator TraM binds to TraR at a site distinct from its DNA-binding motif and induces an allosteric conformational change in the protein, thereby preventing DNA binding. Structural analysis reveals a highly conserved TraR-TraM interface and suggests a mechanism for antiactivation complex formation. This structure may inform alternative strategies to control quorum-sensing-regulated microbial activity including amelioration of infectious disease and antibiotic resistance. In addition, the structural basis of antiactivation presents a regulatory interaction that provides general insights relevant to the field of transcription regulation and signal transduction.
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===Crystal structure of an anti-activation complex in bacterial quorum sensing===
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Structural basis for antiactivation in bacterial quorum sensing.,Chen G, Jeffrey PD, Fuqua C, Shi Y, Chen L Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16474-9. Epub 2007 Oct 5. PMID:17921255<ref>PMID:17921255</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17921255}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2q0o" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17921255 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17921255}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2Q0O is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Rhizobium_sp. Rhizobium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q0O OCA].
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[[Category: Sinorhizobium fredii NGR234]]
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[[Category: Chen G]]
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==Reference==
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[[Category: Chen L]]
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<ref group="xtra">PMID:17921255</ref><references group="xtra"/>
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[[Category: Fuqua C]]
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[[Category: Rhizobium sp.]]
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[[Category: Jeffrey PD]]
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[[Category: Chen, G.]]
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[[Category: Shi Y]]
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[[Category: Chen, L.]]
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[[Category: Fuqua, C.]]
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[[Category: Jeffrey, P D.]]
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[[Category: Shi, Y.]]
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[[Category: Helix-turn-helix]]
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[[Category: Transcription]]
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[[Category: Two-helix coiled coil]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 05:45:15 2009''
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Current revision

Crystal structure of an anti-activation complex in bacterial quorum sensing

PDB ID 2q0o

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