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2aqx

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(New page: 200px<br /><applet load="2aqx" size="450" color="white" frame="true" align="right" spinBox="true" caption="2aqx, resolution 2.50&Aring;" /> '''Crystal Structure of...)
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[[Image:2aqx.gif|left|200px]]<br /><applet load="2aqx" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2aqx, resolution 2.50&Aring;" />
 
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'''Crystal Structure of the Catalytic and CaM-Binding domains of Inositol 1,4,5-Trisphosphate 3-Kinase B'''<br />
 
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==Overview==
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==Crystal Structure of the Catalytic and CaM-Binding domains of Inositol 1,4,5-Trisphosphate 3-Kinase B==
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D-Myoinositol 1,4,5-trisphophate 3-kinases (IP(3)-3Ks) play important, roles in metazoan cellular signaling. It has been demonstrated that mice, without a functional version of IP(3)-3K isoform B are deficient in, peripheral T-cells, indicating that IP(3)-3KB is essential to the, developing immune system. The recent apo IP(3)-3KA structure exhibited a, helix at the catalytic domain N-terminus exhibited a helix at the, N-terminus of the catalytic domain, with a tryptophan indole moiety, mimicking the binding mode of the substrate ATP purine ring, suggesting a, mechanism of autoinhibition. Here we present the structure of the complete, catalytic domain of IP(3)-3KB, including the CaM binding domain in complex, with Mg(2+) and ATP. The crystal structure reveals a homodimeric, arrangement of IP(3)-3KB catalytic domains, mediated via an intermolecular, antiparallel beta-sheet formed from part of the CaM binding region., Residues from the putative autoinhibitory helix are rearranged into a loop, configuration, with extensive interactions with the bound ATP. Mutagenesis, of residues from this region reveals that substitution of the putative, autoinhibitory tryptophan generates a hyperactive enzyme which retains, Ca(2+)/CaM sensitivity. The IP(3)-3KB structure suggests a mechanism of, enzyme activation, and raises the possibility that an interaction between, IP(3)-3KB molecules may occur as part of the catalytic or regulatory, cycle.
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<StructureSection load='2aqx' size='340' side='right'caption='[[2aqx]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2aqx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AQX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AQX FirstGlance]. <br>
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2AQX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with MG and ATP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Inositol-trisphosphate_3-kinase Inositol-trisphosphate 3-kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.127 2.7.1.127] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AQX OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2aqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aqx OCA], [https://pdbe.org/2aqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2aqx RCSB], [https://www.ebi.ac.uk/pdbsum/2aqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2aqx ProSAT]</span></td></tr>
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Structural insights into enzyme regulation for inositol 1,4,5-trisphosphate 3-kinase B., Chamberlain PP, Sandberg ML, Sauer K, Cooke MP, Lesley SA, Spraggon G, Biochemistry. 2005 Nov 8;44(44):14486-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16262249 16262249]
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</table>
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[[Category: Inositol-trisphosphate 3-kinase]]
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== Function ==
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[https://www.uniprot.org/uniprot/B2RXC2_MOUSE B2RXC2_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aq/2aqx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2aqx ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Chamberlain PP]]
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[[Category: Chamberlain, P.P.]]
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[[Category: Cooke MP]]
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[[Category: Cooke, M.P.]]
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[[Category: Lesley SA]]
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[[Category: Lesley, S.A.]]
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[[Category: Sandberg ML]]
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[[Category: Sandberg, M.L.]]
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[[Category: Sauer K]]
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[[Category: Sauer, K.]]
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[[Category: Spraggon G]]
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[[Category: Spraggon, G.]]
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[[Category: ATP]]
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[[Category: MG]]
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[[Category: calmodulin binding]]
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[[Category: inositol]]
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[[Category: ip3]]
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[[Category: ip3-3k]]
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[[Category: ip3-3kb]]
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[[Category: ip3k]]
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[[Category: itpkb]]
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[[Category: kinase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:18:44 2007''
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Current revision

Crystal Structure of the Catalytic and CaM-Binding domains of Inositol 1,4,5-Trisphosphate 3-Kinase B

PDB ID 2aqx

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