2arc

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(New page: 200px<br /><applet load="2arc" size="450" color="white" frame="true" align="right" spinBox="true" caption="2arc, resolution 1.5&Aring;" /> '''ESCHERICHIA COLI REGU...)
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[[Image:2arc.gif|left|200px]]<br /><applet load="2arc" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2arc, resolution 1.5&Aring;" />
 
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'''ESCHERICHIA COLI REGULATORY PROTEIN ARAC COMPLEXED WITH L-ARABINOSE'''<br />
 
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==Overview==
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==ESCHERICHIA COLI REGULATORY PROTEIN ARAC COMPLEXED WITH L-ARABINOSE==
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The crystal structure of the arabinose-binding and dimerization domain of, the Escherchia coli gene regulatory protein AraC was determined in the, presence and absence of L-arabinose. The 1.5 angstrom structure of the, arabinose-bound molecule shows that the protein adopts an unusual fold, binding sugar within a beta barrel and completely burying the arabinose, with the amino-terminal arm of the protein. Dimer contacts in the presence, of arabinose are mediated by an antiparallel coiled-coil. In the 2.8, angstrom structure of the uncomplexed protein, the amino-terminal arm is, disordered, uncovering the sugar-binding pocket and allowing it to serve, as an oligomerization interface. The ligand-gated oligomerization as seen, in AraC provides the basis of a plausible mechanism for modulating the, protein's DNA-looping properties.
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<StructureSection load='2arc' size='340' side='right'caption='[[2arc]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2arc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ARC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ARC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ARA:ALPHA-L-ARABINOSE'>ARA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2arc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2arc OCA], [https://pdbe.org/2arc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2arc RCSB], [https://www.ebi.ac.uk/pdbsum/2arc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2arc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ARAC_ECOLI ARAC_ECOLI] This protein controls the expression of at least six genes that are involved in the transport and catabolism of L-arabinose. It regulates initiation of transcription of the araBAD operon and it also controls its own synthesis. The L-arabinose operon displays both positive and negative regulation through AraC.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ar/2arc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2arc ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2ARC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ARA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2ARC OCA].
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*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural basis for ligand-regulated oligomerization of AraC., Soisson SM, MacDougall-Shackleton B, Schleif R, Wolberger C, Science. 1997 Apr 18;276(5311):421-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9103202 9103202]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Soisson, S.M.]]
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[[Category: Soisson SM]]
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[[Category: Wolberger, C.]]
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[[Category: Wolberger C]]
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[[Category: ARA]]
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[[Category: carbohydrate binding]]
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[[Category: coiled-coil]]
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[[Category: jelly roll]]
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[[Category: transcription factor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:18:59 2007''
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Current revision

ESCHERICHIA COLI REGULATORY PROTEIN ARAC COMPLEXED WITH L-ARABINOSE

PDB ID 2arc

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