2atp

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(New page: 200px<br /><applet load="2atp" size="450" color="white" frame="true" align="right" spinBox="true" caption="2atp, resolution 2.4&Aring;" /> '''Crystal structure of ...)
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[[Image:2atp.gif|left|200px]]<br /><applet load="2atp" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2atp, resolution 2.4&Aring;" />
 
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'''Crystal structure of a CD8ab heterodimer'''<br />
 
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==Overview==
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==Crystal structure of a CD8ab heterodimer==
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The crystal structure of a recombinant mouse single chain CD8alphabeta, ectodomains at 2.4 A resolution reveals paired immunoglobulin variable, region-like domains with a striking resemblance to CD8alphaalpha in size, shape, and surface electrostatic potential of complementarity-determining, regions (CDR), despite &lt;20% sequence identity between the CD8alpha and, CD8beta subunits. Unlike the CD8alpha subunit(s) in the heterodimer or, homodimer, the CDR1 loop of CD8beta tilts away from its corresponding CDR2, and CDR3 loops. Consistent with this observation, independent mutational, studies reveal that alanine substitutions of residues in the CDR1 loop of, CD8beta have no effect on CD8alphabeta coreceptor function, whereas, mutations in CD8beta CDR2 and CDR3 loops abolish CD8alphabeta coreceptor, activity. The implications of these findings and additional CD8alpha, mutational studies for CD8alphabeta- versus CD8alphaalpha-MHCI binding are, discussed.
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<StructureSection load='2atp' size='340' side='right'caption='[[2atp]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2atp]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ATP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ATP FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2atp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2atp OCA], [https://pdbe.org/2atp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2atp RCSB], [https://www.ebi.ac.uk/pdbsum/2atp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2atp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CD8A_MOUSE CD8A_MOUSE] Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/at/2atp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2atp ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of a recombinant mouse single chain CD8alphabeta ectodomains at 2.4 A resolution reveals paired immunoglobulin variable region-like domains with a striking resemblance to CD8alphaalpha in size, shape, and surface electrostatic potential of complementarity-determining regions (CDR), despite &lt;20% sequence identity between the CD8alpha and CD8beta subunits. Unlike the CD8alpha subunit(s) in the heterodimer or homodimer, the CDR1 loop of CD8beta tilts away from its corresponding CDR2 and CDR3 loops. Consistent with this observation, independent mutational studies reveal that alanine substitutions of residues in the CDR1 loop of CD8beta have no effect on CD8alphabeta coreceptor function, whereas mutations in CD8beta CDR2 and CDR3 loops abolish CD8alphabeta coreceptor activity. The implications of these findings and additional CD8alpha mutational studies for CD8alphabeta- versus CD8alphaalpha-MHCI binding are discussed.
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==About this Structure==
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Structural and mutational analyses of a CD8alphabeta heterodimer and comparison with the CD8alphaalpha homodimer.,Chang HC, Tan K, Ouyang J, Parisini E, Liu JH, Le Y, Wang X, Reinherz EL, Wang JH Immunity. 2005 Dec;23(6):661-71. PMID:16356863<ref>PMID:16356863</ref>
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2ATP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with NAG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2ATP OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural and mutational analyses of a CD8alphabeta heterodimer and comparison with the CD8alphaalpha homodimer., Chang HC, Tan K, Ouyang J, Parisini E, Liu JH, Le Y, Wang X, Reinherz EL, Wang JH, Immunity. 2005 Dec;23(6):661-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16356863 16356863]
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</div>
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[[Category: Mus musculus]]
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<div class="pdbe-citations 2atp" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Chang, H.C.]]
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[[Category: Le, Y.]]
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[[Category: Liu, J.H.]]
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[[Category: Ouyang, J.]]
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[[Category: Parisini, E.]]
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[[Category: Reinherz, E.L.]]
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[[Category: Tan, K.]]
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[[Category: Wang, J.H.]]
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[[Category: Wang, X.]]
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[[Category: NAG]]
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[[Category: cd8aa]]
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[[Category: cd8ab]]
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[[Category: mhc]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:22:09 2007''
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==See Also==
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*[[CD8 3D structures|CD8 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mus musculus]]
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[[Category: Chang HC]]
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[[Category: Le Y]]
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[[Category: Liu JH]]
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[[Category: Ouyang J]]
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[[Category: Parisini E]]
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[[Category: Reinherz EL]]
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[[Category: Tan K]]
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[[Category: Wang JH]]
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[[Category: Wang X]]

Current revision

Crystal structure of a CD8ab heterodimer

PDB ID 2atp

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