2ayy

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(New page: 200px<br /><applet load="2ayy" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ayy" /> '''Solution structure of the E.coli RcsC C-term...)
Current revision (11:19, 22 May 2024) (edit) (undo)
 
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[[Image:2ayy.gif|left|200px]]<br /><applet load="2ayy" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ayy" />
 
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'''Solution structure of the E.coli RcsC C-terminus (residues 700-816) containing linker region'''<br />
 
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==Overview==
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==Solution structure of the E.coli RcsC C-terminus (residues 700-816) containing linker region==
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The Rcs signalling pathway controls a variety of physiological functions, like capsule synthesis, cell division or motility in prokaryotes. The Rcs, regulation cascade, involving a multi-step phosphorelay between the two, membrane-bound hybrid sensor kinases RcsC and RcsD and the global, regulator RcsB, is, up to now, one of the most complicated regulatory, systems in bacteria. To understand the structural basis of Rcs signal, transduction, NMR spectroscopy was employed to determine the solution, structure of the RcsC C terminus, possessing a phosphoreceiver domain, (RcsC-PR), and a region previously described as a long linker between the, histidine kinase domain of RcsC (RcsC-HK) and the RcsC-PR. We have found, that the linker region comprises an independent structural domain of a new, alpha/beta organization, which we named RcsC-ABL domain (Alpha/Beta/Loop)., The ABL domain appears to be a conserved and unique structural element of, RcsC-like kinases with no significant sequence homology to other proteins., The second domain of the C terminus, the RcsC-PR domain, represents a, well-folded CheY-like phosphoreceiver domain with the central parallel, beta-sheet covered with two alpha-helical layers on both sides. We have, mapped the interaction of RcsC-ABL and RcsC-PR with the histidine, phosphotransfer domain (HPt) of RcsD. In addition we have characterized, the interaction with and the conformational effects of Mg2+ and the, phosphorylation mimetic BeF(-)(3) on RcsC-ABL and RcsC-PR.
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<StructureSection load='2ayy' size='340' side='right'caption='[[2ayy]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ayy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AYY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ayy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ayy OCA], [https://pdbe.org/2ayy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ayy RCSB], [https://www.ebi.ac.uk/pdbsum/2ayy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ayy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RCSC_ECOLI RCSC_ECOLI] Component of the Rcs signaling system, which controls transcription of numerous genes. RcsC functions as a membrane-associated protein kinase that phosphorylates RcsD in response to environmental signals. The phosphoryl group is then transferred to the response regulator RcsB. RcsC has also phosphatase activity. The system controls expression of genes involved in colanic acid capsule synthesis, biofilm formation and cell division.<ref>PMID:10564486</ref> <ref>PMID:11758943</ref> <ref>PMID:11309126</ref> <ref>PMID:11807084</ref> <ref>PMID:13129944</ref> <ref>PMID:14651646</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ay/2ayy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ayy ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Rcs signalling pathway controls a variety of physiological functions like capsule synthesis, cell division or motility in prokaryotes. The Rcs regulation cascade, involving a multi-step phosphorelay between the two membrane-bound hybrid sensor kinases RcsC and RcsD and the global regulator RcsB, is, up to now, one of the most complicated regulatory systems in bacteria. To understand the structural basis of Rcs signal transduction, NMR spectroscopy was employed to determine the solution structure of the RcsC C terminus, possessing a phosphoreceiver domain (RcsC-PR), and a region previously described as a long linker between the histidine kinase domain of RcsC (RcsC-HK) and the RcsC-PR. We have found that the linker region comprises an independent structural domain of a new alpha/beta organization, which we named RcsC-ABL domain (Alpha/Beta/Loop). The ABL domain appears to be a conserved and unique structural element of RcsC-like kinases with no significant sequence homology to other proteins. The second domain of the C terminus, the RcsC-PR domain, represents a well-folded CheY-like phosphoreceiver domain with the central parallel beta-sheet covered with two alpha-helical layers on both sides. We have mapped the interaction of RcsC-ABL and RcsC-PR with the histidine phosphotransfer domain (HPt) of RcsD. In addition we have characterized the interaction with and the conformational effects of Mg2+ and the phosphorylation mimetic BeF(-)(3) on RcsC-ABL and RcsC-PR.
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==About this Structure==
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A new structural domain in the Escherichia coli RcsC hybrid sensor kinase connects histidine kinase and phosphoreceiver domains.,Rogov VV, Rogova NY, Bernhard F, Koglin A, Lohr F, Dotsch V J Mol Biol. 2006 Nov 17;364(1):68-79. Epub 2006 Jul 29. PMID:17005198<ref>PMID:17005198</ref>
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2AYY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2AYY OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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A new structural domain in the Escherichia coli RcsC hybrid sensor kinase connects histidine kinase and phosphoreceiver domains., Rogov VV, Rogova NY, Bernhard F, Koglin A, Lohr F, Dotsch V, J Mol Biol. 2006 Nov 17;364(1):68-79. Epub 2006 Jul 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17005198 17005198]
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</div>
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<div class="pdbe-citations 2ayy" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bernhard, F.]]
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[[Category: Bernhard F]]
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[[Category: Dotsch, V.]]
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[[Category: Dotsch V]]
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[[Category: Koglin, A.]]
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[[Category: Koglin A]]
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[[Category: Lohr, F.]]
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[[Category: Lohr F]]
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[[Category: Rogov, V.V.]]
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[[Category: Rogov VV]]
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[[Category: Rogova, N.Y.]]
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[[Category: Rogova NY]]
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[[Category: alpha/beta layer]]
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[[Category: new domain]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:28:19 2007''
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Current revision

Solution structure of the E.coli RcsC C-terminus (residues 700-816) containing linker region

PDB ID 2ayy

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