2az1
From Proteopedia
(Difference between revisions)
(New page: 200px<br /><applet load="2az1" size="450" color="white" frame="true" align="right" spinBox="true" caption="2az1, resolution 2.35Å" /> '''Structure of a halop...) |
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| - | [[Image:2az1.gif|left|200px]]<br /><applet load="2az1" size="450" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="2az1, resolution 2.35Å" /> | ||
| - | '''Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum'''<br /> | ||
| - | == | + | ==Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum== |
| - | Nucleoside diphosphate kinase from the halophilic archaeon Halobacterium | + | <StructureSection load='2az1' size='340' side='right'caption='[[2az1]], [[Resolution|resolution]] 2.35Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2az1]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AZ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AZ1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2az1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2az1 OCA], [https://pdbe.org/2az1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2az1 RCSB], [https://www.ebi.ac.uk/pdbsum/2az1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2az1 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/NDK_HALSA NDK_HALSA] Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/az/2az1_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2az1 ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Nucleoside diphosphate kinase from the halophilic archaeon Halobacterium salinarum was crystallized in a free state and a substrate-bound form with CDP. The structures were solved to a resolution of 2.35 and 2.2A, respectively. Crystals with the apo-form were obtained with His6-tagged enzyme, whereas the untagged form was used for co-crystallization with the nucleotide. Crosslinking under different salt and pH conditions revealed a stronger oligomerization tendency for the tagged protein at low and high salt concentrations. The influence of the His6-tag on the halophilic nature of the enzyme is discussed on the basis of the observed structural properties. | ||
| - | + | Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum.,Besir H, Zeth K, Bracher A, Heider U, Ishibashi M, Tokunaga M, Oesterhelt D FEBS Lett. 2005 Dec 5;579(29):6595-600. Epub 2005 Nov 9. PMID:16293253<ref>PMID:16293253</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2az1" style="background-color:#fffaf0;"></div> | |
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| - | + | ==See Also== | |
| + | *[[Nucleoside diphosphate kinase 3D structures|Nucleoside diphosphate kinase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Halobacterium salinarum]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Besir H]] | ||
| + | [[Category: Bracher A]] | ||
| + | [[Category: Heider U]] | ||
| + | [[Category: Ishibashi M]] | ||
| + | [[Category: Oesterhelt D]] | ||
| + | [[Category: Tokunaga M]] | ||
| + | [[Category: Zeth K]] | ||
Current revision
Structure of a halophilic nucleoside diphosphate kinase from Halobacterium salinarum
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