1s97

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{{Seed}}
 
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[[Image:1s97.png|left|200px]]
 
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==DPO4 with GT mismatch==
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The line below this paragraph, containing "STRUCTURE_1s97", creates the "Structure Box" on the page.
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<StructureSection load='1s97' size='340' side='right'caption='[[1s97]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1s97]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S97 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S97 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DCT:2,3-DIDEOXYCYTIDINE+5-TRIPHOSPHATE'>DCT</scene></td></tr>
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{{STRUCTURE_1s97| PDB=1s97 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s97 OCA], [https://pdbe.org/1s97 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s97 RCSB], [https://www.ebi.ac.uk/pdbsum/1s97 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s97 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DPO4_SACS2 DPO4_SACS2] Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. It is involved in translesional synthesis.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s9/1s97_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s97 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The ability or inability of a DNA polymerase to extend a mispair directly affects the establishment of genomic mutations. We report here kinetic analyses of the ability of Dpo4, a Y-family polymerase from Sulfolobus solfataricus, to extend from all mispairs opposite a template G or T. Dpo4 is equally inefficient at extending these mispairs, which include, surprisingly, a G.T mispair expected to conform closely to Watson-Crick geometry. To elucidate the basis of this, we solved the structure of Dpo4 bound to G.T-mispaired primer template in the presence of an incoming nucleotide. As a control, we also determined the structure of Dpo4 bound to a matched A-T base pair at the primer terminus. The structures offer a basis for the low efficiency of Dpo4 in extending a G.T mispair: a reverse wobble that deflects the primer 3'-OH away from the incoming nucleotide.
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===DPO4 with GT mismatch===
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Dpo4 is hindered in extending a G.T mismatch by a reverse wobble.,Trincao J, Johnson RE, Wolfle WT, Escalante CR, Prakash S, Prakash L, Aggarwal AK Nat Struct Mol Biol. 2004 May;11(5):457-62. Epub 2004 Apr 11. PMID:15077104<ref>PMID:15077104</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1s97" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15077104}}, adds the Publication Abstract to the page
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*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15077104 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15077104}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1S97 is a 12 chains structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S97 OCA].
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[[Category: Saccharolobus solfataricus]]
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[[Category: Aggarwal AK]]
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==Reference==
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[[Category: Escalante CR]]
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<ref group="xtra">PMID:15077104</ref><references group="xtra"/>
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[[Category: Johnson RE]]
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[[Category: DNA-directed DNA polymerase]]
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[[Category: Prakash L]]
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[[Category: Sulfolobus solfataricus]]
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[[Category: Prakash S]]
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[[Category: Aggarwal, A K.]]
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[[Category: Trincao J]]
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[[Category: Escalante, C R.]]
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[[Category: Wolfle WT]]
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[[Category: Johnson, R E.]]
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[[Category: Prakash, L.]]
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[[Category: Prakash, S.]]
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[[Category: Trincao, J.]]
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[[Category: Wolfle, W T.]]
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[[Category: Dna duplex]]
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[[Category: G t mismatch]]
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[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 06:49:38 2009''
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Current revision

DPO4 with GT mismatch

PDB ID 1s97

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