3bb0

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{{Seed}}
 
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[[Image:3bb0.png|left|200px]]
 
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==Crystal Structure of a Trapped Phosphate-Intermediate in Vanadium Apochloroperoxidase Catalyzing a Dephosphorylation Reaction==
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The line below this paragraph, containing "STRUCTURE_3bb0", creates the "Structure Box" on the page.
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<StructureSection load='3bb0' size='340' side='right'caption='[[3bb0]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3bb0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Curvularia_inaequalis Curvularia inaequalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BB0 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=PO3:PHOSPHITE+ION'>PO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3bb0| PDB=3bb0 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bb0 OCA], [https://pdbe.org/3bb0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bb0 RCSB], [https://www.ebi.ac.uk/pdbsum/3bb0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bb0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PRXC_CURIN PRXC_CURIN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bb/3bb0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bb0 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the apo form of vanadium chloroperoxidase from Curvularia inaequalis reacted with para-nitrophenylphosphate was determined at a resolution of 1.5 A. The aim of this study was to solve structural details of the dephosphorylation reaction catalyzed by this enzyme. Since the chloroperoxidase is functionally and evolutionary related to several acid phosphatases including human glucose-6-phosphatase and a group of membrane-bound lipid phosphatases, the structure sheds light on the details of the dephosphorylation catalyzed by these enzymes as well. The trapped intermediate found is bound to the active site as a metaphosphate anion PO3-, with its phosphorus atom covalently attached to the Nepsilon2 atom of His496. An apical water molecule is within hydrogen-bonding distance to the phosphorus atom of the metaphosphate, and it is in a perfect position for a nucleophilic attack on the metaphosphate-histidine intermediate to form the inorganic phosphate. This is, to our knowledge, the first structural characterization of a real reaction intermediate of the inorganic phosphate group release in a dephosphorylation reaction.
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===Crystal Structure of a Trapped Phosphate-Intermediate in Vanadium Apochloroperoxidase Catalyzing a Dephosphorylation Reaction===
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Crystal structure of a trapped phosphate intermediate in vanadium apochloroperoxidase catalyzing a dephosphorylation reaction.,Macedo-Ribeiro S, Renirie R, Wever R, Messerschmidt A Biochemistry. 2008 Jan 22;47(3):929-34. Epub 2007 Dec 29. PMID:18163651<ref>PMID:18163651</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3bb0" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18163651}}, adds the Publication Abstract to the page
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*[[Haloperoxidase|Haloperoxidase]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18163651 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18163651}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3BB0 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Curvularia_inaequalis Curvularia inaequalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BB0 OCA].
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==Reference==
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<ref group="xtra">PMID:18163651</ref><ref group="xtra">PMID:8552646</ref><ref group="xtra">PMID:10499093</ref><references group="xtra"/>
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[[Category: Chloride peroxidase]]
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[[Category: Curvularia inaequalis]]
[[Category: Curvularia inaequalis]]
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[[Category: Macedo-Ribeiro, S.]]
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[[Category: Large Structures]]
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[[Category: Messerschmidt, A.]]
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[[Category: Macedo-Ribeiro S]]
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[[Category: Chloride]]
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[[Category: Messerschmidt A]]
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[[Category: Metal-binding]]
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[[Category: Oxidoreductase]]
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[[Category: Peroxidase]]
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[[Category: Phospatase activity]]
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[[Category: Protein phosphate-intermediate complex]]
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[[Category: Secreted]]
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[[Category: Vanadium]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 07:02:14 2009''
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Current revision

Crystal Structure of a Trapped Phosphate-Intermediate in Vanadium Apochloroperoxidase Catalyzing a Dephosphorylation Reaction

PDB ID 3bb0

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