2b3w

Structural highlights

Function

RIBX_ECOLI Catalyzes the hydrolysis of the N-glycosidic bond in the first two intermediates of riboflavin biosynthesis, which are highly reactive metabolites, yielding relatively innocuous products. Thus, can divert a surplus of harmful intermediates into relatively harmless products and pre-empt the damage these intermediates would otherwise do. Helps maintain flavin levels. May act on other substrates in vivo. Has no activity against GTP, nucleoside monophosphates or ADP-ribose (PubMed:25431972). Is Required for swarming motility (PubMed:17122336).^{[1] [2]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

1. ↑ Inoue T, Shingaki R, Hirose S, Waki K, Mori H, Fukui K. Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. J Bacteriol. 2007 Feb;189(3):950-7. PMID:17122336 doi:10.1128/JB.01294-06
2. ↑ Frelin O, Huang L, Hasnain G, Jeffryes JG, Ziemak MJ, Rocca JR, Wang B, Rice J, Roje S, Yurgel SN, Gregory JF 3rd, Edison AS, Henry CS, de Crécy-Lagard V, Hanson AD. A directed-overflow and damage-control N-glycosidase in riboflavin biosynthesis. Biochem J. 2015 Feb 15;466(1):137-45. PMID:25431972 doi:10.1042/BJ20141237