1kte

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{{Seed}}
 
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[[Image:1kte.png|left|200px]]
 
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==CRYSTAL STRUCTURE OF THIOLTRANSFERASE AT 2.2 ANGSTROM RESOLUTION==
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The line below this paragraph, containing "STRUCTURE_1kte", creates the "Structure Box" on the page.
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<StructureSection load='1kte' size='340' side='right'caption='[[1kte]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1kte]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KTE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KTE FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kte FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kte OCA], [https://pdbe.org/1kte PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kte RCSB], [https://www.ebi.ac.uk/pdbsum/1kte PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kte ProSAT]</span></td></tr>
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{{STRUCTURE_1kte| PDB=1kte | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GLRX1_PIG GLRX1_PIG] Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kt/1kte_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kte ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We report here the first three-dimensional structure of a mammalian thioltransferase as determined by single crystal X-ray crystallography at 2.2 A resolution. The protein is known for its thiol-redox properties and dehydroascorbate reductase activity. Recombinant pig liver thioltransferase expressed in Escherichia coli was crystallized in its oxidized form by vapor diffusion technique. The structure was determined by multiple isomorphous replacement method using four heavy-atom derivatives. The protein folds into an alpha/beta structure with a four-stranded mixed beta-sheet in the core, flanked on either side by helices. The fold is similar to that found in other thiol-redox proteins, viz. E. coli thioredoxin and bacteriophage T4 glutaredoxin, and thus seems to be conserved in these functionally related proteins. The active site disulfide (Cys 22-Cys 25) is located on a protrusion on the molecular surface. Cys 22, which is known to have an abnormally low pKa of 3.8, is accessible from the exterior of the molecule. Pro 70, which is in close proximity to the disulfide bridge, assumes a conserved cis-peptide configuration. Mutational data available on the protein are in agreement with the three-dimensional structure.
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===CRYSTAL STRUCTURE OF THIOLTRANSFERASE AT 2.2 ANGSTROM RESOLUTION===
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Crystal structure of thioltransferase at 2.2 A resolution.,Katti SK, Robbins AH, Yang Y, Wells WW Protein Sci. 1995 Oct;4(10):1998-2005. PMID:8535236<ref>PMID:8535236</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_8535236}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1kte" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 8535236 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_8535236}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1KTE is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KTE OCA].
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==Reference==
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<ref group="xtra">PMID:8535236</ref><references group="xtra"/>
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[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Katti, S K.]]
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[[Category: Katti SK]]
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[[Category: Robbins, A H.]]
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[[Category: Robbins AH]]
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[[Category: Acetylation]]
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[[Category: Electron transport]]
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[[Category: Redox-active center]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 07:39:41 2009''
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Current revision

CRYSTAL STRUCTURE OF THIOLTRANSFERASE AT 2.2 ANGSTROM RESOLUTION

PDB ID 1kte

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