2b4q

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(New page: 200px<br /><applet load="2b4q" size="450" color="white" frame="true" align="right" spinBox="true" caption="2b4q, resolution 2.30&Aring;" /> '''Pseudomonas aerugino...)
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[[Image:2b4q.gif|left|200px]]<br /><applet load="2b4q" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2b4q, resolution 2.30&Aring;" />
 
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'''Pseudomonas aeruginosa RhlG/NADP active-site complex'''<br />
 
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==Overview==
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==Pseudomonas aeruginosa RhlG/NADP active-site complex==
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Rhamnolipids are extracellular biosurfactants and virulence factors, secreted by the opportunistic human pathogen Pseudomonas aeruginosa that, are required for swarming motility. The rhlG gene is essential for, rhamnolipid formation, and the RhlG enzyme is thought to divert fatty acid, synthesis intermediates into the rhamnolipid biosynthetic pathway based on, its similarity to FabG, the beta-ketoacyl-acyl carrier protein (ACP), reductase of type II fatty acid synthesis. Crystallographic analysis, reveals that the overall structures of the RhlG.NADP+ and FabG.NADP+, complexes are indeed similar, but there are key differences related to, function. RhlG does not undergo the conformational changes upon NADP(H), binding at the active site that in FabG are the structural basis of, negative allostery. Also, the acyl chain-binding pocket of RhlG is narrow, and rigid compared with the larger, flexible substrate-binding subdomain, in FabG. Finally, RhlG lacks a positively charged/hydrophobic surface, feature adjacent to the active site that is found on enzymes like FabG, that recognize the ACP of fatty acid synthesis. RhlG catalyzed the, NADPH-dependent reduction of beta-ketodecanoyl-ACP to, beta-d-hydroxydecanoyl-ACP. However, the enzyme was 2000-fold less active, than FabG in carrying out the same reaction. These structural and, biochemical studies establish RhlG as a NADPH-dependent beta-ketoacyl, reductase of the SDR protein superfamily and further suggest that the ACP, of fatty acid synthesis does not carry the substrates for RhlG.
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<StructureSection load='2b4q' size='340' side='right'caption='[[2b4q]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2b4q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B4Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B4Q FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b4q OCA], [https://pdbe.org/2b4q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b4q RCSB], [https://www.ebi.ac.uk/pdbsum/2b4q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b4q ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RHLG_PSEAE RHLG_PSEAE] Required for the synthesis of the beta-hydroxy acid moiety of rhamnolipids.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b4/2b4q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b4q ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2B4Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with NAP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2B4Q OCA].
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*[[Beta-ketoacyl carrier protein reductase 3D structures|Beta-ketoacyl carrier protein reductase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of RhlG, an essential beta-ketoacyl reductase in the rhamnolipid biosynthetic pathway of Pseudomonas aeruginosa., Miller DJ, Zhang YM, Rock CO, White SW, J Biol Chem. 2006 Jun 30;281(26):18025-32. Epub 2006 Apr 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16624803 16624803]
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[[Category: Large Structures]]
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[[Category: 3-oxoacyl-[acyl-carrier-protein] reductase]]
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[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Single protein]]
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[[Category: Miller DJ]]
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[[Category: Miller, D.J.]]
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[[Category: White SW]]
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[[Category: White, S.W.]]
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[[Category: NAP]]
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[[Category: pseudomonas aeruginosa]]
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[[Category: rhlg-nadp complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:33:51 2007''
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Current revision

Pseudomonas aeruginosa RhlG/NADP active-site complex

PDB ID 2b4q

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