1hkh

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{{Seed}}
 
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[[Image:1hkh.png|left|200px]]
 
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==unligated gamma lactamase from an Aureobacterium species==
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The line below this paragraph, containing "STRUCTURE_1hkh", creates the "Structure Box" on the page.
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<StructureSection load='1hkh' size='340' side='right'caption='[[1hkh]], [[Resolution|resolution]] 1.73&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1hkh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Microbacterium Microbacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HKH FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.73&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1hkh| PDB=1hkh | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hkh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hkh OCA], [https://pdbe.org/1hkh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hkh RCSB], [https://www.ebi.ac.uk/pdbsum/1hkh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hkh ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8GJP7_9MICO Q8GJP7_9MICO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hk/1hkh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hkh ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of the recombinant (-) gamma-lactamase from an Aureobacterium species has been solved at 1.73A resolution in the cubic space group F23 with unit cell parameters a=b=c=240.6A. The trimeric enzyme has an alpha/beta hydrolase fold and closely resembles the cofactor free haloperoxidases. The structure has been solved in complex with a covalently bound ligand originating from the host cell and also in the unligated form. The associated density in the former structure has been interpreted as the two-ring ligand (3aR,7aS)-3a,4,7,7a-tetrahydro-benzo [1,3] dioxol-2-one which forms a tetrahedral complex with OG of the catalytic Ser98. Soaks of these crystals with the industrial substrate gamma-lactam or its structural analogue, norcamphor, result in the displacement of the ligand from the enzyme active site, thereby allowing determination of the unligated structure. The presence of the ligand in the active site protects the enzyme from serine hydrolase inhibitors. Cyclic ethylene carbonate, the first ring of the ligand, was shown to be a substrate of the enzyme.
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===UNLIGATED GAMMA LACTAMASE FROM AN AUREOBACTERIUM SPECIES===
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The crystal structure of a (-) gamma-lactamase from an Aureobacterium species reveals a tetrahedral intermediate in the active site.,Line K, Isupov MN, Littlechild JA J Mol Biol. 2004 Apr 30;338(3):519-32. PMID:15081810<ref>PMID:15081810</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15081810}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1hkh" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15081810 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15081810}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1HKH is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Microbacterium Microbacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKH OCA].
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==Reference==
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<ref group="xtra">PMID:15081810</ref><references group="xtra"/>
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[[Category: Microbacterium]]
[[Category: Microbacterium]]
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[[Category: Isupov, M N.]]
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[[Category: Isupov MN]]
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[[Category: Line, K.]]
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[[Category: Line K]]
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[[Category: Littlechild, J A.]]
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[[Category: Littlechild JA]]
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[[Category: Alpha/beta hydrolase]]
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[[Category: Co-factor free haloperoxidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 07:55:39 2009''
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Current revision

unligated gamma lactamase from an Aureobacterium species

PDB ID 1hkh

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