5eau

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{{Seed}}
 
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[[Image:5eau.png|left|200px]]
 
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==5-EPI-ARISTOLOCHENE SYNTHASE FROM NICOTIANA TABACUM==
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The line below this paragraph, containing "STRUCTURE_5eau", creates the "Structure Box" on the page.
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<StructureSection load='5eau' size='340' side='right'caption='[[5eau]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[5eau]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EAU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EAU FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FFF:TRIFLUOROFURNESYL+DIPHOSPHATE'>FFF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_5eau| PDB=5eau | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eau OCA], [https://pdbe.org/5eau PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eau RCSB], [https://www.ebi.ac.uk/pdbsum/5eau PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eau ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/5EAS_TOBAC 5EAS_TOBAC] Catalyzes the cyclization of trans,trans-farnesyl diphosphate (FPP) to the bicyclic intermediate 5-epi-aristolochene, initial step in the conversion of FPP to the sesquiterpenoid antifungal phytoalexin capsidiol. Produces germacrene A as an enzyme-bound intermediate that is not released by the enzyme, but is further cyclized to produce the bicyclic 5-epi-aristolochene.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ea/5eau_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=5eau ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Terpene cyclases catalyze the synthesis of cyclic terpenes with 10-, 15-, and 20-carbon acyclic isoprenoid diphosphates as substrates. Plants have been a source of these natural products by providing a homologous set of terpene synthases. The crystal structures of 5-epi-aristolochene synthase, a sesquiterpene cyclase from tobacco, alone and complexed separately with two farnesyl diphosphate analogs were analyzed. These structures reveal an unexpected enzymatic mechanism for the synthesis of the bicyclic product, 5-epi-aristolochene, and provide a basis for understanding the stereochemical selectivity displayed by other cyclases in the biosynthesis of pharmacologically important cyclic terpenes. As such, these structures provide templates for the engineering of novel terpene cyclases.
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===5-EPI-ARISTOLOCHENE SYNTHASE FROM NICOTIANA TABACUM===
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Structural basis for cyclic terpene biosynthesis by tobacco 5-epi-aristolochene synthase.,Starks CM, Back K, Chappell J, Noel JP Science. 1997 Sep 19;277(5333):1815-20. PMID:9295271<ref>PMID:9295271</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_9295271}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 5eau" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 9295271 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_9295271}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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5EAU is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EAU OCA].
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==Reference==
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<ref group="xtra">PMID:9295271</ref><references group="xtra"/>
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[[Category: Nicotiana tabacum]]
[[Category: Nicotiana tabacum]]
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[[Category: Back, K.]]
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[[Category: Back K]]
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[[Category: Chappell, J.]]
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[[Category: Chappell J]]
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[[Category: Noel, J P.]]
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[[Category: Noel JP]]
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[[Category: Starks, C M.]]
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[[Category: Starks CM]]
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[[Category: 5-epi-aristolochene synthase]]
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[[Category: Isoprenoid cyclase]]
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[[Category: Isoprenoid synthase]]
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[[Category: Natural products biosynthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 08:02:22 2009''
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Current revision

5-EPI-ARISTOLOCHENE SYNTHASE FROM NICOTIANA TABACUM

PDB ID 5eau

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