2f3o

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{{Seed}}
 
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[[Image:2f3o.png|left|200px]]
 
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==Crystal Structure of a glycyl radical enzyme from Archaeoglobus fulgidus==
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The line below this paragraph, containing "STRUCTURE_2f3o", creates the "Structure Box" on the page.
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<StructureSection load='2f3o' size='340' side='right'caption='[[2f3o]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2f3o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F3O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F3O FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
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{{STRUCTURE_2f3o| PDB=2f3o | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f3o OCA], [https://pdbe.org/2f3o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f3o RCSB], [https://www.ebi.ac.uk/pdbsum/2f3o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f3o ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O28823_ARCFU O28823_ARCFU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f3/2f3o_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f3o ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We have solved the crystal structure of a PFL2 from Archaeglobus fulgidus at 2.9 A resolution. Of the three previously solved enzyme structures of glycyl radical enzymes, pyruvate formate lyase (PFL), anaerobic ribonucleotide reductase and glycerol dehydratase (GD), the last one is clearly most similar to PFL2. We observed electron density in the active site of PFL2, which we modelled as glycerol. The orientation of the glycerol is different from that in GD, and changes in the active site indicate that the actual substrate of PFL2 is bigger than a glycerol molecule, but sequence and structural homology suggest that PFL2 may be a dehydratase. Crystal packing, solution X-ray scattering and ultracentrifugation experiments show that PFL2 is tetrameric, unlike other glycyl radical enzymes. A.fulgidus is a hyperthermophile and PFL2 appears to be stabilized by several factors including an increased number of ion pairs, differences in buried charges, a truncated N terminus, anchoring of loops and N terminus via salt-bridges, changes in the oligomeric interface and perhaps also the higher oligomerization state of the protein.
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===Crystal Structure of a glycyl radical enzyme from Archaeoglobus fulgidus===
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Crystal structure of a glycyl radical enzyme from Archaeoglobus fulgidus.,Lehtio L, Grossmann JG, Kokona B, Fairman R, Goldman A J Mol Biol. 2006 Mar 17;357(1):221-35. Epub 2006 Jan 3. PMID:16414072<ref>PMID:16414072</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16414072}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2f3o" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16414072 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16414072}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2F3O is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F3O OCA].
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==Reference==
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<ref group="xtra">PMID:16414072</ref><references group="xtra"/>
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[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
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[[Category: Goldman, A.]]
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[[Category: Large Structures]]
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[[Category: Lehtio, L.]]
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[[Category: Goldman A]]
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[[Category: Glycerol dehydratase]]
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[[Category: Lehtio L]]
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[[Category: Glycyl radical]]
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[[Category: Hyperthermophilic]]
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[[Category: Pfl2]]
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[[Category: Pyruvate formate lyase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 08:08:16 2009''
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Current revision

Crystal Structure of a glycyl radical enzyme from Archaeoglobus fulgidus

PDB ID 2f3o

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