2jp7

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{{Seed}}
 
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[[Image:2jp7.png|left|200px]]
 
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==NMR structure of the Mex67 UBA domain==
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The line below this paragraph, containing "STRUCTURE_2jp7", creates the "Structure Box" on the page.
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<StructureSection load='2jp7' size='340' side='right'caption='[[2jp7]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2jp7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JP7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JP7 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jp7 OCA], [https://pdbe.org/2jp7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jp7 RCSB], [https://www.ebi.ac.uk/pdbsum/2jp7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jp7 ProSAT]</span></td></tr>
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{{STRUCTURE_2jp7| PDB=2jp7 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MEX67_YEAST MEX67_YEAST] Involved in the export of mRNA from the nucleus to the cytoplasm.<ref>PMID:9214641</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jp/2jp7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jp7 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The ubiquitin-associated (UBA) domain of the mRNA nuclear export receptor Mex67 helps in coordinating transcription elongation and nuclear export by interacting both with ubiquitin conjugates and specific targets, such as Hpr1, a component of the THO complex. Here, we analyzed substrate specificity and ubiquitin selectivity of the Mex67 UBA domain. UBA-Mex67 is formed by three helices arranged in a classical UBA fold plus a fourth helix, H4. Deletion or mutation of helix H4 strengthens the interaction between UBA-Mex67 and ubiquitin, but it decreases its affinity for Hpr1. Interaction with Hpr1 is required for Mex67 UBA domain to bind polyubiquitin, possibly by inducing an H4-dependent conformational change. In vivo, deletion of helix H4 reduces cotranscriptional recruitment of Mex67 on activated genes, and it also shows an mRNA export defect. Based on these results, we propose that H4 functions as a molecular switch that coordinates the interaction of Mex67 with ubiquitin bound to specific substrates, defines the selectivity of the Mex67 UBA domain for polyubiquitin, and prevents its binding to nonspecific substrates.
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===NMR structure of the Mex67 UBA domain===
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Coordination of Hpr1 and ubiquitin binding by the UBA domain of the mRNA export factor Mex67.,Hobeika M, Brockmann C, Iglesias N, Gwizdek C, Neuhaus D, Stutz F, Stewart M, Divita G, Dargemont C Mol Biol Cell. 2007 Jul;18(7):2561-8. Epub 2007 May 2. PMID:17475778<ref>PMID:17475778</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17475778}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2jp7" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17475778 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17475778}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2JP7 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JP7 OCA].
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==Reference==
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<ref group="xtra">PMID:17475778</ref><references group="xtra"/>
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Brockmann, C.]]
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[[Category: Brockmann C]]
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[[Category: Dargemont, C.]]
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[[Category: Dargemont C]]
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[[Category: Divita, G.]]
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[[Category: Divita G]]
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[[Category: Gwizdek, C.]]
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[[Category: Gwizdek C]]
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[[Category: Hobeika, M.]]
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[[Category: Hobeika M]]
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[[Category: Iglesias, N.]]
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[[Category: Iglesias N]]
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[[Category: Neuhaus, D.]]
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[[Category: Neuhaus D]]
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[[Category: Stewart, M.]]
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[[Category: Stewart M]]
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[[Category: Stutz, F.]]
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[[Category: Stutz F]]
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[[Category: Mex67]]
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[[Category: Solution nmr]]
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[[Category: Uba]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 08:09:51 2009''
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Current revision

NMR structure of the Mex67 UBA domain

PDB ID 2jp7

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OCA

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