2gas

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{{Seed}}
 
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[[Image:2gas.png|left|200px]]
 
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==Crystal Structure of Isoflavone Reductase==
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The line below this paragraph, containing "STRUCTURE_2gas", creates the "Structure Box" on the page.
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<StructureSection load='2gas' size='340' side='right'caption='[[2gas]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2gas]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GAS FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gas OCA], [https://pdbe.org/2gas PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gas RCSB], [https://www.ebi.ac.uk/pdbsum/2gas PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gas ProSAT]</span></td></tr>
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{{STRUCTURE_2gas| PDB=2gas | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IFR_MEDSA IFR_MEDSA] Reduces achiral isoflavones to chiral isoflavanones during the biosynthesis of chiral pterocarpan phytoalexins. The reduction product is a third isomer, which represents the penultimate intermediate in the synthesis of the phytoalexin (-)-medicarpin, the major phytoalexin in Alfalfa.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ga/2gas_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gas ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Isoflavonoids play important roles in plant defense and exhibit a range of mammalian health-promoting activities. Isoflavone reductase (IFR) specifically recognizes isoflavones and catalyzes a stereospecific NADPH-dependent reduction to (3R)-isoflavanone. The crystal structure of Medicago sativa IFR with deletion of residues 39-47 has been determined at 1.6A resolution. Structural analysis, molecular modeling and docking, and comparison with the structures of other NADPH-dependent enzymes, defined the putative binding sites for co-factor and substrate and potential key residues for enzyme activity and substrate specificity. Further mutagenesis has confirmed the role of Lys144 as a catalytic residue. This study provides a structural basis for understanding the enzymatic mechanism and substrate specificity of IFRs as well as the functions of IFR-like proteins.
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===Crystal Structure of Isoflavone Reductase===
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Crystal structure of isoflavone reductase from alfalfa (Medicago sativa L.).,Wang X, He X, Lin J, Shao H, Chang Z, Dixon RA J Mol Biol. 2006 May 19;358(5):1341-52. Epub 2006 Mar 29. PMID:16600295<ref>PMID:16600295</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16600295}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2gas" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16600295 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16600295}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2GAS is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GAS OCA].
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==Reference==
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<ref group="xtra">PMID:16600295</ref><references group="xtra"/>
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[[Category: Medicago sativa]]
[[Category: Medicago sativa]]
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[[Category: Chang, Z.]]
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[[Category: Chang Z]]
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[[Category: Dixon, R A.]]
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[[Category: Dixon RA]]
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[[Category: He, X.]]
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[[Category: He X]]
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[[Category: Lin, J.]]
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[[Category: Lin J]]
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[[Category: Shao, H.]]
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[[Category: Shao H]]
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[[Category: Wang, X.]]
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[[Category: Wang X]]
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[[Category: Nadph-dependent reductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 08:13:42 2009''
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Current revision

Crystal Structure of Isoflavone Reductase

PDB ID 2gas

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