2pee

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{{Seed}}
 
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[[Image:2pee.png|left|200px]]
 
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==Crystal Structure of a Thermophilic Serpin, Tengpin, in the Native State==
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The line below this paragraph, containing "STRUCTURE_2pee", creates the "Structure Box" on the page.
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<StructureSection load='2pee' size='340' side='right'caption='[[2pee]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2pee]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldanaerobacter_subterraneus_subsp._tengcongensis_MB4 Caldanaerobacter subterraneus subsp. tengcongensis MB4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PEE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2pee| PDB=2pee | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pee OCA], [https://pdbe.org/2pee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pee RCSB], [https://www.ebi.ac.uk/pdbsum/2pee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pee ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8R9P5_CALS4 Q8R9P5_CALS4]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pe/2pee_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pee ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Serpins fold to a metastable native state and are susceptible to undergoing spontaneous conformational change to more stable conformers, such as the latent form. We investigated conformational change in tengpin, an unusual prokaryotic serpin from the extremophile Thermoanaerobacter tengcongensis. In addition to the serpin domain, tengpin contains a functionally uncharacterized 56-amino-acid amino-terminal region. Deletion of this domain creates a variant--tengpinDelta51--which folds past the native state and readily adopts the latent conformation. Analysis of crystal structures together with mutagenesis studies show that the N terminus of tengpin protects a hydrophobic patch in the serpin domain and functions to trap tengpin in its native metastable state. A 13-amino-acid peptide derived from the N terminus is able to mimick the role of the N terminus in stabilizing the native state of tengpinDelta51. Therefore, the function of the N terminus in tengpin resembles protein cofactors that prevent mammalian serpins from spontaneously adopting the latent conformation.
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===Crystal Structure of a Thermophilic Serpin, Tengpin, in the Native State===
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The N terminus of the serpin, tengpin, functions to trap the metastable native state.,Zhang Q, Buckle AM, Law RH, Pearce MC, Cabrita LD, Lloyd GJ, Irving JA, Smith AI, Ruzyla K, Rossjohn J, Bottomley SP, Whisstock JC EMBO Rep. 2007 Jul;8(7):658-63. Epub 2007 Jun 8. PMID:17557112<ref>PMID:17557112</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17557112}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2pee" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17557112 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17557112}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Caldanaerobacter subterraneus subsp. tengcongensis MB4]]
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2PEE is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Thermoanaerobacter_tengcongensis Thermoanaerobacter tengcongensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PEE OCA].
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[[Category: Large Structures]]
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[[Category: Buckle AM]]
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==Reference==
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[[Category: Whisstock JC]]
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<ref group="xtra">PMID:17557112</ref><references group="xtra"/>
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[[Category: Zhang QW]]
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[[Category: Thermoanaerobacter tengcongensis]]
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[[Category: Buckle, A M.]]
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[[Category: Whisstock, J C.]]
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[[Category: Zhang, Q W.]]
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[[Category: Thermophilic serpin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 08:21:00 2009''
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Current revision

Crystal Structure of a Thermophilic Serpin, Tengpin, in the Native State

PDB ID 2pee

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