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3bjx

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{{Seed}}
 
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[[Image:3bjx.png|left|200px]]
 
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==Structure of a Group I haloacid dehalogenase from Pseudomonas putida strain PP3==
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The line below this paragraph, containing "STRUCTURE_3bjx", creates the "Structure Box" on the page.
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<StructureSection load='3bjx' size='340' side='right'caption='[[3bjx]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3bjx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BJX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BJX FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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-->
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3bjx| PDB=3bjx | SCENE= }}
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dehI ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 "Bacillus fluorescens putidus" Flugge 1886])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.2 3.8.1.2] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bjx OCA], [https://pdbe.org/3bjx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bjx RCSB], [https://www.ebi.ac.uk/pdbsum/3bjx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bjx ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bj/3bjx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bjx ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Haloacid dehalogenases catalyse the removal of halides from organic haloacids and are of interest for bioremediation and for their potential use in the synthesis of industrial chemicals. We present the crystal structure of the homodimer DehI from Pseudomonas putida strain PP3, the first structure of a group I alpha-haloacid dehalogenase that can process both L- and D-substrates. The structure shows that the DehI monomer consists of two domains of approximately 130 amino acids that have approximately 16% sequence identity yet adopt virtually identical and unique folds that form a pseudo-dimer. Analysis of the active site reveals the likely binding mode of both L- and D-substrates with respect to key catalytic residues. Asp189 is predicted to activate a water molecule for nucleophilic attack of the substrate chiral centre resulting in an inversion of configuration of either l- or d-substrates in contrast to D-only enzymes. These details will assist with future bioengineering of dehalogenases.
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===Structure of a Group I haloacid dehalogenase from Pseudomonas putida strain PP3===
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The crystal structure of DehI reveals a new alpha-haloacid dehalogenase fold and active-site mechanism.,Schmidberger JW, Wilce JA, Weightman AJ, Whisstock JC, Wilce MC J Mol Biol. 2008 Apr 18;378(1):284-94. Epub 2008 Feb 29. PMID:18353360<ref>PMID:18353360</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3bjx" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18353360}}, adds the Publication Abstract to the page
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*[[Dehalogenase 3D structures|Dehalogenase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18353360 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18353360}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Bacillus fluorescens putidus flugge 1886]]
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3BJX is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BJX OCA].
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==Reference==
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<ref group="xtra">PMID:18353360</ref><references group="xtra"/>
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[[Category: Pseudomonas putida]]
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[[Category: Schmidberger, J W.]]
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[[Category: Wilce, M C.J.]]
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
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[[Category: Large Structures]]
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[[Category: Schmidberger, J W]]
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[[Category: Wilce, M C.J]]
[[Category: Plasmid]]
[[Category: Plasmid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 09:06:53 2009''
 

Current revision

Structure of a Group I haloacid dehalogenase from Pseudomonas putida strain PP3

PDB ID 3bjx

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