2bi6

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(New page: 200px<br /><applet load="2bi6" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bi6" /> '''NMR STUDY OF BROMELAIN INHIBITOR VI FROM PIN...)
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[[Image:2bi6.jpg|left|200px]]<br /><applet load="2bi6" size="450" color="white" frame="true" align="right" spinBox="true"
 
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'''NMR STUDY OF BROMELAIN INHIBITOR VI FROM PINEAPPLE STEM'''<br />
 
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==Overview==
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==NMR STUDY OF BROMELAIN INHIBITOR VI FROM PINEAPPLE STEM==
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Bromelain inhibitor VI from pineapple stem (BI-VI) is a unique, double-chain inhibitor with an 11-residue light chain and a 41-residue, heavy chain by disulfide bonds and inhibits the cysteine proteinase, bromelain competitively. The structure of BI-VI in aqueous solution was, determined using nuclear magnetic resonance spectroscopy and simulated, annealing-based calculations. Its three-dimensional structure was shown to, be composed of two distinct domains, each of which is formed by a, three-stranded antiparallel beta-sheet. Unexpectedly, BI-VI was found to, share a similar folding and disulfide bond connectivities not with, cystatin superfamily inhibitors which inhibit the same cysteine, proteinases but with the Bowman-Birk trypsin/chymotrypsin inhibitor from, soybean (BBI-I). BBI-I is a 71-residue inhibitor which has two independent, inhibitory sites toward the serine proteinases trypsin and chymotrypsin., These structural similarities with BBI-I suggest that they have evolved, from a common ancestor and differentiated in function during a course of, molecular evolution.
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<StructureSection load='2bi6' size='340' side='right'caption='[[2bi6]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2bi6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ananas_comosus Ananas comosus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BI6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BI6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 18 models</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bi6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bi6 OCA], [https://pdbe.org/2bi6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bi6 RCSB], [https://www.ebi.ac.uk/pdbsum/2bi6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bi6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IBRO_ANACO IBRO_ANACO] Weak inhibitor of cysteine proteinases.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bromelain inhibitor VI from pineapple stem (BI-VI) is a unique double-chain inhibitor with an 11-residue light chain and a 41-residue heavy chain by disulfide bonds and inhibits the cysteine proteinase bromelain competitively. The structure of BI-VI in aqueous solution was determined using nuclear magnetic resonance spectroscopy and simulated annealing-based calculations. Its three-dimensional structure was shown to be composed of two distinct domains, each of which is formed by a three-stranded antiparallel beta-sheet. Unexpectedly, BI-VI was found to share a similar folding and disulfide bond connectivities not with cystatin superfamily inhibitors which inhibit the same cysteine proteinases but with the Bowman-Birk trypsin/chymotrypsin inhibitor from soybean (BBI-I). BBI-I is a 71-residue inhibitor which has two independent inhibitory sites toward the serine proteinases trypsin and chymotrypsin. These structural similarities with BBI-I suggest that they have evolved from a common ancestor and differentiated in function during a course of molecular evolution.
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==About this Structure==
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Solution structure of bromelain inhibitor IV from pineapple stem: structural similarity with Bowman-Birk trypsin/chymotrypsin inhibitor from soybean.,Hatano K, Kojima M, Tanokura M, Takahashi K Biochemistry. 1996 Apr 30;35(17):5379-84. PMID:8611527<ref>PMID:8611527</ref>
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2BI6 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Ananas_comosus Ananas comosus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BI6 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Solution structure of bromelain inhibitor IV from pineapple stem: structural similarity with Bowman-Birk trypsin/chymotrypsin inhibitor from soybean., Hatano K, Kojima M, Tanokura M, Takahashi K, Biochemistry. 1996 Apr 30;35(17):5379-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8611527 8611527]
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</div>
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<div class="pdbe-citations 2bi6" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Ananas comosus]]
[[Category: Ananas comosus]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Hatano, K.I.]]
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[[Category: Hatano K-I]]
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[[Category: cysteine protease inhibitor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:47:52 2007''
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NMR STUDY OF BROMELAIN INHIBITOR VI FROM PINEAPPLE STEM

PDB ID 2bi6

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