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2bjv

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(New page: 200px<br /><applet load="2bjv" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bjv, resolution 1.70&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:2bjv.gif|left|200px]]<br /><applet load="2bjv" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2bjv, resolution 1.70&Aring;" />
 
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'''CRYSTAL STRUCTURE OF PSPF(1-275) R168A MUTANT'''<br />
 
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==Overview==
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==Crystal Structure of PspF(1-275) R168A mutant==
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Activators of bacterial sigma54-RNA polymerase holoenzyme are, mechanochemical proteins that use adenosine triphosphate (ATP) hydrolysis, to activate transcription. We have determined by cryogenic electron, microscopy (cryo-EM) a 20 angstrom resolution structure of an activator, phage shock protein F [PspF(1-275)], which is bound to an ATP transition, state analog in complex with its basal factor, sigma54. By fitting the, crystal structure of PspF(1-275) at 1.75 angstroms into the EM map, we, identified two loops involved in binding sigma54. Comparing, enhancer-binding structures in different nucleotide states and mutational, analysis led us to propose nucleotide-dependent conformational changes, that free the loops for association with sigma54.
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<StructureSection load='2bjv' size='340' side='right'caption='[[2bjv]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2bjv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BJV FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjv OCA], [https://pdbe.org/2bjv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bjv RCSB], [https://www.ebi.ac.uk/pdbsum/2bjv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bjv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PSPF_ECOLI PSPF_ECOLI] Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene.<ref>PMID:8606168</ref> <ref>PMID:15485810</ref> <ref>PMID:19804784</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bj/2bjv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bjv ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Activators of bacterial sigma54-RNA polymerase holoenzyme are mechanochemical proteins that use adenosine triphosphate (ATP) hydrolysis to activate transcription. We have determined by cryogenic electron microscopy (cryo-EM) a 20 angstrom resolution structure of an activator, phage shock protein F [PspF(1-275)], which is bound to an ATP transition state analog in complex with its basal factor, sigma54. By fitting the crystal structure of PspF(1-275) at 1.75 angstroms into the EM map, we identified two loops involved in binding sigma54. Comparing enhancer-binding structures in different nucleotide states and mutational analysis led us to propose nucleotide-dependent conformational changes that free the loops for association with sigma54.
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==About this Structure==
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Structural insights into the activity of enhancer-binding proteins.,Rappas M, Schumacher J, Beuron F, Niwa H, Bordes P, Wigneshweraraj S, Keetch CA, Robinson CV, Buck M, Zhang X Science. 2005 Mar 25;307(5717):1972-5. PMID:15790859<ref>PMID:15790859</ref>
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2BJV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BJV OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural insights into the activity of enhancer-binding proteins., Rappas M, Schumacher J, Beuron F, Niwa H, Bordes P, Wigneshweraraj S, Keetch CA, Robinson CV, Buck M, Zhang X, Science. 2005 Mar 25;307(5717):1972-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15790859 15790859]
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</div>
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<div class="pdbe-citations 2bjv" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Beuron, F.]]
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[[Category: Beuron F]]
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[[Category: Bordes, P.]]
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[[Category: Bordes P]]
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[[Category: Buck, M.]]
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[[Category: Buck M]]
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[[Category: Keetch, C.A.]]
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[[Category: Keetch CA]]
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[[Category: Niwa, H.]]
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[[Category: Niwa H]]
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[[Category: Rappas, M.]]
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[[Category: Rappas M]]
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[[Category: Robinson, C.V.]]
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[[Category: Robinson CV]]
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[[Category: Schumacher, J.]]
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[[Category: Schumacher J]]
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[[Category: Wigneshweraraj, S.]]
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[[Category: Wigneshweraraj S]]
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[[Category: Zhang, X.]]
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[[Category: Zhang X]]
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[[Category: aaa]]
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[[Category: atp-binding]]
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[[Category: dna-binding]]
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[[Category: enhancer binding protein]]
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[[Category: gene regulation]]
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[[Category: pspf]]
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[[Category: sigma54 activator]]
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[[Category: transcription activation]]
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[[Category: transcription regulation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:48:29 2007''
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Current revision

Crystal Structure of PspF(1-275) R168A mutant

PDB ID 2bjv

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