2iu4

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:26, 9 April 2025) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2iu4.png|left|200px]]
 
-
<!--
+
==Dihydroxyacetone kinase operon co-activator Dha-DhaQ==
-
The line below this paragraph, containing "STRUCTURE_2iu4", creates the "Structure Box" on the page.
+
<StructureSection load='2iu4' size='340' side='right'caption='[[2iu4]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2iu4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IU4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IU4 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.96&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HIQ:1-[1,2-DIHYDROXY-1-(HYDROXYMETHYL)ETHYL]-L-HISTIDINE'>HIQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
{{STRUCTURE_2iu4| PDB=2iu4 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iu4 OCA], [https://pdbe.org/2iu4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iu4 RCSB], [https://www.ebi.ac.uk/pdbsum/2iu4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iu4 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/DHAQ_LACLA DHAQ_LACLA] Coactivator for the transcription factor DhaS. The heterotetramer formed by DhaQ and DhaS functions as a transcriptional regulator. Activated by covalent binding of dihydroxyacetone to DhaQ. The complex activates the dhaKLM operon.<ref>PMID:16760471</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iu/2iu4_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iu4 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Dihydroxyacetone (Dha) kinases are a novel family of kinases with signaling and metabolic functions. Here we report the x-ray structures of the transcriptional activator DhaS and the coactivator DhaQ and characterize their function. DhaQ is a paralog of the Dha binding Dha kinase subunit; DhaS belongs to the family of TetR repressors although, unlike all known members of this family, it is a transcriptional activator. DhaQ and DhaS form a stable complex that in the presence of Dha activates transcription of the Lactococcus lactis dha operon. Dha covalently binds to DhaQ through a hemiaminal bond with a histidine and thereby induces a conformational change, which is propagated to the surface via a cantilever-like structure. DhaS binding protects an inverted repeat whose sequence is GGACACATN6ATTTGTCC and renders two GC base pairs of the operator DNA hypersensitive to DNase I cleavage. The proximal half-site of the inverted repeat partially overlaps with the predicted -35 consensus sequence of the dha promoter.
-
===DIHYDROXYACETONE KINASE OPERON CO-ACTIVATOR DHA-DHAQ===
+
Regulation of the Dha operon of Lactococcus lactis: a deviation from the rule followed by the Tetr family of transcription regulators.,Christen S, Srinivas A, Bahler P, Zeller A, Pridmore D, Bieniossek C, Baumann U, Erni B J Biol Chem. 2006 Aug 11;281(32):23129-37. Epub 2006 Jun 7. PMID:16760471<ref>PMID:16760471</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_16760471}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 2iu4" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 16760471 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_16760471}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
2IU4 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Lactococcus_lactis Lactococcus lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IU4 OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:16760471</ref><references group="xtra"/>
+
-
[[Category: Glucokinase]]
+
[[Category: Lactococcus lactis]]
[[Category: Lactococcus lactis]]
-
[[Category: Baumann, U.]]
+
[[Category: Large Structures]]
-
[[Category: Christen, S.]]
+
[[Category: Baumann U]]
-
[[Category: Erni, B.]]
+
[[Category: Christen S]]
-
[[Category: Srinivas, A.]]
+
[[Category: Erni B]]
-
[[Category: Co-activator]]
+
[[Category: Srinivas A]]
-
[[Category: Dihydroxyacetone]]
+
-
[[Category: Kinase]]
+
-
[[Category: Lactococcus lacti]]
+
-
[[Category: Transferase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 09:17:43 2009''
+

Current revision

Dihydroxyacetone kinase operon co-activator Dha-DhaQ

PDB ID 2iu4

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2iu4"
Personal tools