2fmd

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:29, 30 August 2023) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2fmd.png|left|200px]]
 
-
<!--
+
==Structural basis of carbohydrate recognition by Bowringia milbraedii seed agglutinin==
-
The line below this paragraph, containing "STRUCTURE_2fmd", creates the "Structure Box" on the page.
+
<StructureSection load='2fmd' size='340' side='right'caption='[[2fmd]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2fmd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leucomphalos_mildbraedii Leucomphalos mildbraedii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FMD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FMD FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASX:ASP/ASN+AMBIGUOUS'>ASX</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PRD_900111:2alpha-alpha-mannobiose'>PRD_900111</scene></td></tr>
-
{{STRUCTURE_2fmd| PDB=2fmd | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fmd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fmd OCA], [https://pdbe.org/2fmd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fmd RCSB], [https://www.ebi.ac.uk/pdbsum/2fmd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fmd ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/LEC_LEUMI LEC_LEUMI] Binds preferentially to oligosaccharides bearing the sequence Man-alpha-1->2 Man-alpha-1->6 Man-alpha-1->6Man found in early steps of glycoprotein processing in the endoplasmic reticulum. It binds weakly to highly processed oligosaccharide structures.<ref>PMID:2925660</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fm/2fmd_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fmd ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The crystal structure of the seed lectin from the tropical legume Bowringia milbraedii was determined in complex with the disaccharide ligand Man(alpha1-2)Man. In solution, the protein exhibits a dynamic dimer-tetramer equilibrium, consistent with the concanavalin A-type tetramer observed in the crystal. Contacts between the tetramers are mediated almost exclusively through the carbohydrate ligand, resulting in a crystal lattice virtually identical to that of the concanavalin-A:Man(alpha1-2)Man complex, even though both proteins have less than 50% sequence identity. The disaccharide binds exclusively in a "downstream" binding mode, with the non-reducing mannose occupying the monosaccharide-binding site. The reducing mannose is bound in a predominantly polar subsite involving Tyr131, Gln218, and Tyr219.
-
===Structural basis of carbohydrate recognition by Bowringia milbraedii seed agglutinin===
+
Structural basis of carbohydrate recognition by a Man(alpha1-2)Man-specific lectin from Bowringia milbraedii.,Buts L, Garcia-Pino A, Wyns L, Loris R Glycobiology. 2006 Jul;16(7):635-40. Epub 2006 Mar 27. PMID:16567368<ref>PMID:16567368</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_16567368}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 2fmd" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 16567368 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_16567368}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
2FMD is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bowringia_mildbraedii Bowringia mildbraedii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FMD OCA].
+
[[Category: Leucomphalos mildbraedii]]
-
 
+
[[Category: Buts L]]
-
==Reference==
+
[[Category: Garcia-Pino A]]
-
<ref group="xtra">PMID:16567368</ref><references group="xtra"/>
+
[[Category: Loris R]]
-
[[Category: Bowringia mildbraedii]]
+
[[Category: Wyns L]]
-
[[Category: Buts, L.]]
+
-
[[Category: Garcia-Pino, A.]]
+
-
[[Category: Loris, R.]]
+
-
[[Category: Wyns, L.]]
+
-
[[Category: Beta sandwich]]
+
-
[[Category: Lectin]]
+
-
[[Category: Legume lectin]]
+
-
[[Category: Protein-carbohydrate complex]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 09:19:35 2009''
+

Current revision

Structural basis of carbohydrate recognition by Bowringia milbraedii seed agglutinin

PDB ID 2fmd

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools