2blm

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(New page: 200px<br /><applet load="2blm" size="450" color="white" frame="true" align="right" spinBox="true" caption="2blm, resolution 2.0&Aring;" /> '''BETA-LACTAMASE OF BAC...)
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[[Image:2blm.jpg|left|200px]]<br /><applet load="2blm" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2blm, resolution 2.0&Aring;" />
 
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'''BETA-LACTAMASE OF BACILLUS LICHENIFORMIS 749(SLASH)C AT 2 ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==BETA-LACTAMASE OF BACILLUS LICHENIFORMIS 749(SLASH)C AT 2 ANGSTROMS RESOLUTION==
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Two crystal forms (A and B) of the 29,500 Da Class A beta-lactamase, (penicillinase) from Bacillus licheniformis 749/C have been examined, crystallographically. The structure of B-form crystals has been solved to, 2 A resolution, the starting model for which was a 3.5 A structure, obtained from A-form crystals. The beta-lactamase has an alpha + beta, structure with 11 helices and 5 beta-strands seen also in a penicillin, target DD-peptidase of Streptomyces R61. Atomic parameters of the two, molecules in the asymmetric unit were refined by simulated annealing at, 2.0 A resolution. The R factor is 0.208 for the 27,330 data greater than 3, sigma (F), with water molecules excluded from the model. The catalytic, Ser-70 is at the N-terminus of a helix and is within hydrogen bonding, distance of conserved Lys-73. Also interacting with the Lys-73 are Asn-132, and the conserved Glu-166, which is on a potentially flexible, helix-containing loop. The structure suggests the binding of beta-lactam, substrates is facilitated by interactions with Lys-234, Thr-235, and, Ala-237 in a conserved beta-strand peptide, which is antiparallel to the, beta-lactam's acylamido linkage; an exposed cavity near Asn-170 exists for, acylamido substituents. The reactive double bond of clavulanate-type, inhibitors may interact with Arg-244 on the fourth beta-strand. A very, similar binding site architecture is seen in the DD-peptidase.
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<StructureSection load='2blm' size='340' side='right'caption='[[2blm]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2blm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BLM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BLM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2blm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2blm OCA], [https://pdbe.org/2blm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2blm RCSB], [https://www.ebi.ac.uk/pdbsum/2blm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2blm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BLAC_BACLI BLAC_BACLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bl/2blm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2blm ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2BLM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Active as [http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BLM OCA].
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*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Beta-lactamase of Bacillus licheniformis 749/C at 2 A resolution., Moews PC, Knox JR, Dideberg O, Charlier P, Frere JM, Proteins. 1990;7(2):156-71. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2326252 2326252]
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[[Category: Bacillus licheniformis]]
[[Category: Bacillus licheniformis]]
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[[Category: Beta-lactamase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Dideberg O]]
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[[Category: Dideberg, O.]]
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[[Category: Knox JR]]
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[[Category: Knox, J.R.]]
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[[Category: Moews PC]]
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[[Category: Moews, P.C.]]
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[[Category: hydrolase(acting in cyclic amides)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 08:49:41 2007''
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Current revision

BETA-LACTAMASE OF BACILLUS LICHENIFORMIS 749(SLASH)C AT 2 ANGSTROMS RESOLUTION

PDB ID 2blm

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