2bib

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(New page: 200px<br /> <applet load="2bib" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bib, resolution 1.92&Aring;" /> '''CRYSTAL STRUCTURE O...)
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[[Image:2bib.gif|left|200px]]<br />
 
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<applet load="2bib" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2bib, resolution 1.92&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE COMPLETE MODULAR TEICHIOIC ACID PHOSPHORYLCHOLINE ESTERASE PCE (CBPE) FROM STREPTOCOCCUS PNEUMONIAE'''<br />
 
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==Overview==
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==Crystal structure of the complete modular teichioic acid phosphorylcholine esterase Pce (CbpE) from Streptococcus pneumoniae==
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Phosphorylcholine, a specific component of the pneumococcal cell wall, is, crucial in pathogenesis. It directly binds to the human, platelet-activating factor (PAF) receptor and acts as a docking station, for the family of surface-located choline-binding proteins (CBP). The, first structure of a complete pneumococcal CBP, Pce (or CbpE), has been, solved in complex with the reaction product and choline analogs. Pce has a, novel modular structure, with a globular N-terminal module containing a, binuclear Zn(2+) catalytic center, and an elongated choline-binding, module. Residues involved in substrate binding and catalysis are described, and modular configuration of the active center accounts for in vivo, features of teichoic acid hydrolysis. The hydrolysis of PAF by Pce and its, regulatory ... [[http://ispc.weizmann.ac.il/pmbin/getpm?15895092 (full description)]]
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<StructureSection load='2bib' size='340' side='right'caption='[[2bib]], [[Resolution|resolution]] 1.92&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2bib]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BIB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BIB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.92&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PC:PHOSPHOCHOLINE'>PC</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bib OCA], [https://pdbe.org/2bib PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bib RCSB], [https://www.ebi.ac.uk/pdbsum/2bib PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bib ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8DQ62_STRR6 Q8DQ62_STRR6]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bi/2bib_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bib ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Phosphorylcholine, a specific component of the pneumococcal cell wall, is crucial in pathogenesis. It directly binds to the human platelet-activating factor (PAF) receptor and acts as a docking station for the family of surface-located choline-binding proteins (CBP). The first structure of a complete pneumococcal CBP, Pce (or CbpE), has been solved in complex with the reaction product and choline analogs. Pce has a novel modular structure, with a globular N-terminal module containing a binuclear Zn(2+) catalytic center, and an elongated choline-binding module. Residues involved in substrate binding and catalysis are described and modular configuration of the active center accounts for in vivo features of teichoic acid hydrolysis. The hydrolysis of PAF by Pce and its regulatory role in phosphorylcholine decoration of the bacterial surface provide new insights into the critical function of Pce in pneumococcal adherence and invasiveness.
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==About this Structure==
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Insights into pneumococcal pathogenesis from the crystal structure of the modular teichoic acid phosphorylcholine esterase Pce.,Hermoso JA, Lagartera L, Gonzalez A, Stelter M, Garcia P, Martinez-Ripoll M, Garcia JL, Menendez M Nat Struct Mol Biol. 2005 Jun;12(6):533-8. Epub 2005 May 15. PMID:15895092<ref>PMID:15895092</ref>
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2BIB is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]] with ZN, CA, PC and BTB as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BIB OCA]].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Insights into pneumococcal pathogenesis from the crystal structure of the modular teichoic acid phosphorylcholine esterase Pce., Hermoso JA, Lagartera L, Gonzalez A, Stelter M, Garcia P, Martinez-Ripoll M, Garcia JL, Menendez M, Nat Struct Mol Biol. 2005 Jun;12(6):533-8. Epub 2005 May 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15895092 15895092]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 2bib" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptococcus pneumoniae]]
[[Category: Streptococcus pneumoniae]]
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[[Category: Garcia, J.L.]]
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[[Category: Garcia JL]]
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[[Category: Garcia, P.]]
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[[Category: Garcia P]]
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[[Category: Gonzalez, A.]]
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[[Category: Gonzalez A]]
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[[Category: Hermoso, J.A.]]
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[[Category: Hermoso JA]]
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[[Category: Lagartera, L.]]
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[[Category: Lagartera L]]
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[[Category: Martinez-Ripoll, M.]]
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[[Category: Martinez-Ripoll M]]
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[[Category: Menendez, M.]]
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[[Category: Menendez M]]
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[[Category: BTB]]
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[[Category: CA]]
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[[Category: PC]]
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[[Category: ZN]]
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[[Category: cbpe]]
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[[Category: choline-binding protein]]
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[[Category: hydrolase]]
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[[Category: pce]]
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[[Category: phosphorylcholine esterase]]
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[[Category: teichoic acid]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 21:48:41 2007''
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Current revision

Crystal structure of the complete modular teichioic acid phosphorylcholine esterase Pce (CbpE) from Streptococcus pneumoniae

PDB ID 2bib

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