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2cdh

From Proteopedia

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(New page: 200px<br /><applet load="2cdh" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cdh, resolution 4.20&Aring;" /> '''ARCHITECTURE OF THE ...)
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[[Image:2cdh.gif|left|200px]]<br /><applet load="2cdh" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2cdh, resolution 4.20&Aring;" />
 
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'''ARCHITECTURE OF THE THERMOMYCES LANUGINOSUS FUNGAL FATTY ACID SYNTHASE AT 5 ANGSTROM RESOLUTION.'''<br />
 
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==Overview==
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==ARCHITECTURE OF THE THERMOMYCES LANUGINOSUS FUNGAL FATTY ACID SYNTHASE AT 5 ANGSTROM RESOLUTION.==
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All steps of fatty acid synthesis in fungi are catalyzed by the fatty acid, synthase, which forms a 2.6-megadalton alpha6beta6 complex. We have, determined the molecular architecture of this multienzyme by fitting the, structures of homologous enzymes that catalyze the individual steps of the, reaction pathway into a 5 angstrom x-ray crystallographic electron density, map. The huge assembly contains two separated reaction chambers, each, equipped with three sets of active sites separated by distances up to, approximately 130 angstroms, across which acyl carrier protein shuttles, substrates during the reaction cycle. Regions of the electron density, arising from well-defined structural features outside the catalytic, domains separate the two reaction chambers and serve as a matrix in which, domains carrying the various active sites are embedded. The structure, rationalizes the compartmentalization of fatty acid synthesis, and the, spatial arrangement of the active sites has specific implications for our, understanding of the reaction cycle mechanism and of the architecture of, multienzymes in general.
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<StructureSection load='2cdh' size='340' side='right'caption='[[2cdh]], [[Resolution|resolution]] 4.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2cdh]] is a 36 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermomyces_lanuginosus Thermomyces lanuginosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CDH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CDH FirstGlance]. <br>
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2CDH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermomyces_lanuginosus Thermomyces lanuginosus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CDH OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cdh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cdh OCA], [https://pdbe.org/2cdh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cdh RCSB], [https://www.ebi.ac.uk/pdbsum/2cdh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cdh ProSAT]</span></td></tr>
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==Reference==
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</table>
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Architecture of a fungal fatty acid synthase at 5 A resolution., Jenni S, Leibundgut M, Maier T, Ban N, Science. 2006 Mar 3;311(5765):1263-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16513976 16513976]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/FABB_ECOLI FABB_ECOLI] Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Specific for elongation from C-10 to unsaturated C-16 and C-18 fatty acids.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cd/2cdh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cdh ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermomyces lanuginosus]]
[[Category: Thermomyces lanuginosus]]
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[[Category: Ban, N.]]
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[[Category: Ban N]]
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[[Category: Jenni, S.]]
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[[Category: Jenni S]]
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[[Category: Leibundgut, M.]]
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[[Category: Leibundgut M]]
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[[Category: Maier, T.]]
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[[Category: Maier T]]
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[[Category: fatty acid synthesis]]
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[[Category: fungal fatty acid synthase]]
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[[Category: multifunctional enzyme]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 09:05:29 2007''
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Current revision

ARCHITECTURE OF THE THERMOMYCES LANUGINOSUS FUNGAL FATTY ACID SYNTHASE AT 5 ANGSTROM RESOLUTION.

PDB ID 2cdh

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