1f2l

From Proteopedia

(Difference between revisions)

Current revision

CRYSTAL STRUCTURE OF CHEMOKINE DOMAIN OF FRACTALKINE

Structural highlights

1f2l is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

X3CL1_HUMAN The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Fractalkine, or neurotactin, is a chemokine that is present in endothelial cells from several tissues, including brain, liver, and kidney. It is the only member of the CX(3)C class of chemokines. Fractalkine contains a chemokine domain (CDF) attached to a membrane-spanning domain via a mucin-like stalk. However, fractalkine can also be proteolytically cleaved from its membrane-spanning domain to release a freely diffusible form. Fractalkine attracts and immobilizes leukocytes by binding to its receptor, CX(3)CR1. The x-ray crystal structure of CDF has been solved and refined to 2.0 A resolution. The CDF monomers form a dimer through an intermolecular beta-sheet. This interaction is somewhat similar to that seen in other dimeric CC chemokine crystal structures. However, the displacement of the first disulfide in CDF causes the dimer to assume a more compact quaternary structure relative to CC chemokines, which is unique to CX(3)C chemokines. Although fractalkine can bind to heparin in vitro, as shown by comparison of electrostatic surface plots with other chemokines and by heparin chromatography, the role of this property in vivo is not well understood.

The crystal structure of the chemokine domain of fractalkine shows a novel quaternary arrangement.,Hoover DM, Mizoue LS, Handel TM, Lubkowski J J Biol Chem. 2000 Jul 28;275(30):23187-93. PMID:10770945^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Xue X, Lu Q, Wei H, Wang D, Chen D, He G, Huang L, Wang H, Wang X. Structural basis of chemokine sequestration by CrmD, a poxvirus-encoded tumor necrosis factor receptor. PLoS Pathog. 2011 Jul;7(7):e1002162. Epub 2011 Jul 28. PMID:21829356 doi:10.1371/journal.ppat.1002162
↑ Hoover DM, Mizoue LS, Handel TM, Lubkowski J. The crystal structure of the chemokine domain of fractalkine shows a novel quaternary arrangement. J Biol Chem. 2000 Jul 28;275(30):23187-93. PMID:10770945 doi:http://dx.doi.org/10.1074/jbc.M002584200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1f2l"

@@ Line 1: / Line 1: @@
-{{Seed}}
-[[Image:1f2l.png|left|200px]]
-<!--
+==CRYSTAL STRUCTURE OF CHEMOKINE DOMAIN OF FRACTALKINE==
-The line below this paragraph, containing "STRUCTURE_1f2l", creates the "Structure Box" on the page.
+<StructureSection load='1f2l' size='340' side='right'caption='[[1f2l]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1f2l]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F2L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F2L FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f2l OCA], [https://pdbe.org/1f2l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f2l RCSB], [https://www.ebi.ac.uk/pdbsum/1f2l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f2l ProSAT]</span></td></tr>
-{{STRUCTURE_1f2l|  PDB=1f2l  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/X3CL1_HUMAN X3CL1_HUMAN] The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.<ref>PMID:21829356</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f2/1f2l_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f2l ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Fractalkine, or neurotactin, is a chemokine that is present in endothelial cells from several tissues, including brain, liver, and kidney. It is the only member of the CX(3)C class of chemokines. Fractalkine contains a chemokine domain (CDF) attached to a membrane-spanning domain via a mucin-like stalk. However, fractalkine can also be proteolytically cleaved from its membrane-spanning domain to release a freely diffusible form. Fractalkine attracts and immobilizes leukocytes by binding to its receptor, CX(3)CR1. The x-ray crystal structure of CDF has been solved and refined to 2.0 A resolution. The CDF monomers form a dimer through an intermolecular beta-sheet. This interaction is somewhat similar to that seen in other dimeric CC chemokine crystal structures. However, the displacement of the first disulfide in CDF causes the dimer to assume a more compact quaternary structure relative to CC chemokines, which is unique to CX(3)C chemokines. Although fractalkine can bind to heparin in vitro, as shown by comparison of electrostatic surface plots with other chemokines and by heparin chromatography, the role of this property in vivo is not well understood.
-===CRYSTAL STRUCTURE OF CHEMOKINE DOMAIN OF FRACTALKINE===
+The crystal structure of the chemokine domain of fractalkine shows a novel quaternary arrangement.,Hoover DM, Mizoue LS, Handel TM, Lubkowski J J Biol Chem. 2000 Jul 28;275(30):23187-93. PMID:10770945<ref>PMID:10770945</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<!--
+</div>
-The line below this paragraph, {{ABSTRACT_PUBMED_10770945}}, adds the Publication Abstract to the page
+<div class="pdbe-citations 1f2l" style="background-color:#fffaf0;"></div>
-(as it appears on PubMed at http://www.pubmed.gov), where 10770945 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_10770945}}
+__TOC__
+</StructureSection>
-==About this Structure==
-F2L is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F2L OCA].
-==Reference==
-<ref group="xtra">PMID:10770945</ref><references group="xtra"/>
 [[Category: Homo sapiens]]
-[[Category: Handel, T M.]]
+[[Category: Large Structures]]
-[[Category: Hoover, D M.]]
+[[Category: Handel TM]]
-[[Category: Lubkowski, J.]]
+[[Category: Hoover DM]]
-[[Category: Mizoue, L S.]]
+[[Category: Lubkowski J]]
-[[Category: Chemoattractant]]
+[[Category: Mizoue LS]]
-[[Category: Fractalkine]]
-[[Category: Neurotactin]]
-[[Category: X-ray structure]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 10:47:13 2009''

1f2l

From Proteopedia

Current revision

CRYSTAL STRUCTURE OF CHEMOKINE DOMAIN OF FRACTALKINE

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox