1lj2

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{{Seed}}
 
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[[Image:1lj2.png|left|200px]]
 
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==Recognition of eIF4G by Rotavirus NSP3 reveals a basis for mRNA circularization==
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The line below this paragraph, containing "STRUCTURE_1lj2", creates the "Structure Box" on the page.
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<StructureSection load='1lj2' size='340' side='right'caption='[[1lj2]], [[Resolution|resolution]] 2.38&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1lj2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Simian_rotavirus_A/SA11 Simian rotavirus A/SA11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LJ2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LJ2 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.38&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AU:GOLD+ION'>AU</scene></td></tr>
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{{STRUCTURE_1lj2| PDB=1lj2 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lj2 OCA], [https://pdbe.org/1lj2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lj2 RCSB], [https://www.ebi.ac.uk/pdbsum/1lj2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lj2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NSP3_ROTS1 NSP3_ROTS1] Involved in the shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lj/1lj2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lj2 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Rotaviruses, segmented double-stranded RNA viruses, co-opt the eukaryotic translation machinery with the aid of nonstructural protein 3 (NSP3), a rotaviral functional homolog of the cellular poly(A) binding protein (PABP). NSP3 binds to viral mRNA 3' consensus sequences and circularizes mRNA via interactions with eIF4G. Here, we present the X-ray structure of the C-terminal domain of NSP3 (NSP3-C) recognizing a fragment of eIF4GI. Homodimerization of NSP3-C yields a symmetric, elongated, largely alpha-helical structure with two hydrophobic eIF4G binding pockets at the dimer interface. Site-directed mutagenesis and isothermal titration calorimetry documented that NSP3 and PABP use analogous eIF4G recognition strategies, despite marked differences in tertiary structure.
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===Recognition of eIF4G by Rotavirus NSP3 reveals a basis for mRNA circularization===
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Recognition of eIF4G by rotavirus NSP3 reveals a basis for mRNA circularization.,Groft CM, Burley SK Mol Cell. 2002 Jun;9(6):1273-83. PMID:12086624<ref>PMID:12086624</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1lj2" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_12086624}}, adds the Publication Abstract to the page
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*[[Nonstructural protein 3D structures|Nonstructural protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 12086624 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12086624}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Homo sapiens]]
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1LJ2 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Simian_rotavirus_a/sa11 Simian rotavirus a/sa11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LJ2 OCA].
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[[Category: Large Structures]]
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[[Category: Simian rotavirus A/SA11]]
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==Reference==
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[[Category: Burley SK]]
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<ref group="xtra">PMID:12086624</ref><references group="xtra"/>
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[[Category: Groft CM]]
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[[Category: Simian rotavirus a/sa11]]
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[[Category: Burley, S K.]]
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[[Category: Groft, C M.]]
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[[Category: Closed loop]]
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[[Category: Coiled coil]]
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[[Category: Eif4g]]
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[[Category: Homodimer]]
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[[Category: Mrna]]
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[[Category: Nsp3]]
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[[Category: Rotavirus]]
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[[Category: Translation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 11:02:30 2009''
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Current revision

Recognition of eIF4G by Rotavirus NSP3 reveals a basis for mRNA circularization

PDB ID 1lj2

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