1lnm

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{{Seed}}
 
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[[Image:1lnm.png|left|200px]]
 
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==ANTICALIN DIGA16 IN COMPLEX WITH DIGITOXIGENIN==
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The line below this paragraph, containing "STRUCTURE_1lnm", creates the "Structure Box" on the page.
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<StructureSection load='1lnm' size='340' side='right'caption='[[1lnm]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1lnm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pieris_brassicae Pieris brassicae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LNM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LNM FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTX:DIGITOXIGENIN'>DTX</scene></td></tr>
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{{STRUCTURE_1lnm| PDB=1lnm | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lnm OCA], [https://pdbe.org/1lnm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lnm RCSB], [https://www.ebi.ac.uk/pdbsum/1lnm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lnm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BBP_PIEBR BBP_PIEBR] This protein binds the blue pigments bilins.<ref>PMID:3202956</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ln/1lnm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lnm ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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DigA16 is an artificial digoxigenin-binding protein, which was derived from the bilin-binding protein, a lipocalin of Pieris brassicae, via reshaping of its natural ligand pocket. Here we report the crystal structures of DigA16 in the presence of either digoxigenin or digitoxigenin and for the apo-protein at resolutions below 1.9A. As a consequence of the altogether 17 amino acid substitutions within the binding site significant structural changes have occurred in the four loops that form the entrance to the ligand pocket on top of the structurally conserved beta-barrel framework. For example, one loop adopts a new alpha-helical backbone structure, which seems to be induced by few critical side-chain contacts. Digoxigenin becomes almost fully buried (by 95%) upon complexation, whereby specificity for the hydrophilic steroid is maintained through hydrogen-bonding networks and shape complementarity. The differential binding of the related steroid digitoxigenin is mainly governed by an internal histidine residue, whose side-chain undergoes significant induced fit. Among those amino acids that line the ligand pocket two tyrosine and one tryptophan residue provide the largest contacts. Interestingly, corresponding three side-chains are found with the same mutual orientation in the anti-digoxigenin antibody 26-10, even though the hapten orientation is quite different there and only 66% of the steroid surface is buried in the combining site. Hence, in the case of the engineered lipocalin DigA16 an example of convergent in vitro evolution is observed. Generally, the remarkable structural plasticity of the loop region and the role of polar residues in the binding site illustrate the potential of the lipocalin scaffold for the generation of specific receptor proteins towards a variety of ligands.
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===ANTICALIN DIGA16 IN COMPLEX WITH DIGITOXIGENIN===
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Structural mechanism of specific ligand recognition by a lipocalin tailored for the complexation of digoxigenin.,Korndorfer IP, Schlehuber S, Skerra A J Mol Biol. 2003 Jul 4;330(2):385-96. PMID:12823976<ref>PMID:12823976</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_12823976}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1lnm" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12823976 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12823976}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1LNM is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Pieris_brassicae Pieris brassicae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LNM OCA].
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==Reference==
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<ref group="xtra">PMID:12823976</ref><references group="xtra"/>
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[[Category: Pieris brassicae]]
[[Category: Pieris brassicae]]
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[[Category: Korndoerfer, I P.]]
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[[Category: Korndoerfer IP]]
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[[Category: Skerra, A.]]
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[[Category: Skerra A]]
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[[Category: Anticalin]]
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[[Category: Digitoxigenin]]
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[[Category: Digoxigenin]]
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[[Category: Genetical engineering]]
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[[Category: Lipocalin]]
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[[Category: Pieris brassicae]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 11:03:08 2009''
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Current revision

ANTICALIN DIGA16 IN COMPLEX WITH DIGITOXIGENIN

PDB ID 1lnm

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