2v7n
From Proteopedia
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| - | {{Seed}} | ||
| - | [[Image:2v7n.png|left|200px]] | ||
| - | < | + | ==Unusual twinning in crystals of the CitS binding antibody Fab fragment f3p4== |
| - | + | <StructureSection load='2v7n' size='340' side='right'caption='[[2v7n]], [[Resolution|resolution]] 1.92Å' scene=''> | |
| - | You may | + | == Structural highlights == |
| - | + | <table><tr><td colspan='2'>[[2v7n]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V7N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V7N FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.92Å</td></tr> | |
| - | - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v7n OCA], [https://pdbe.org/2v7n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v7n RCSB], [https://www.ebi.ac.uk/pdbsum/2v7n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v7n ProSAT]</span></td></tr> |
| - | + | </table> | |
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v7/2v7n_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2v7n ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The structure of the antibody Fab fragment f3p4, which was selected from a subset of the synthetic HuCAL antibody library to bind the sodium citrate symporter CitS, is described at 1.92 A resolution. Comparison with computational models revealed deviations in a few framework positions and in the binding loops. The crystals belong to space group P2(1)2(1)2 and contain four molecules in the asymmetric unit, with unit-cell parameters a=102.77, b=185.92, c=102.97 A. These particular unit-cell parameters allowed pseudo-merohedral twinning; interestingly, the twinning law relates a twofold screw axis to a twofold axis. | ||
| - | + | Structure of the recombinant antibody Fab fragment f3p4.,Frey D, Huber T, Pluckthun A, Grutter MG Acta Crystallogr D Biol Crystallogr. 2008 Jun;64(Pt 6):636-43. Epub 2008 May 14. PMID:18560151<ref>PMID:18560151</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2v7n" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Synthetic construct]] |
| - | [[Category: | + | [[Category: Frey D]] |
| - | [[Category: | + | [[Category: Gruetter MG]] |
| - | + | [[Category: Huber T]] | |
| - | + | [[Category: Plueckthun A]] | |
Current revision
Unusual twinning in crystals of the CitS binding antibody Fab fragment f3p4
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