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1hh1

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{{Seed}}
 
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[[Image:1hh1.png|left|200px]]
 
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==THE STRUCTURE OF HJC, A HOLLIDAY JUNCTION RESOLVING ENZYME FROM SULFOLOBUS SOLFATARICUS==
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The line below this paragraph, containing "STRUCTURE_1hh1", creates the "Structure Box" on the page.
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<StructureSection load='1hh1' size='340' side='right'caption='[[1hh1]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1hh1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HH1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HH1 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hh1 OCA], [https://pdbe.org/1hh1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hh1 RCSB], [https://www.ebi.ac.uk/pdbsum/1hh1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hh1 ProSAT]</span></td></tr>
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{{STRUCTURE_1hh1| PDB=1hh1 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HJC_SACS2 HJC_SACS2] A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination; may have some degree of sequence preference in a mobile junction. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products. Can cleave all 4 strands 3 bases 3' of the junction center. Cleaves both mobile and immobile junctions. Modifies the structure of the 4-way DNA junction, a model Holliday junction structure. The protein forms multiple complexes with 4-way DNA, suggesting more than 1 homodimer can bind to each junction.<ref>PMID:10701121</ref> <ref>PMID:10736227</ref> <ref>PMID:10940317</ref> <ref>PMID:11709558</ref> <ref>PMID:17011573</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/1hh1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hh1 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The 2.15-A structure of Hjc, a Holliday junction-resolving enzyme from the archaeon Sulfolobus solfataricus, reveals extensive structural homology with a superfamily of nucleases that includes type II restriction enzymes. Hjc is a dimer with a large DNA-binding surface consisting of numerous basic residues surrounding the metal-binding residues of the active sites. Residues critical for catalysis, identified on the basis of sequence comparisons and site-directed mutagenesis studies, are clustered to produce two active sites in the dimer, about 29 A apart, consistent with the requirement for the introduction of paired nicks in opposing strands of the four-way DNA junction substrate. Hjc displays similarity to the restriction endonucleases in the way its specific DNA-cutting pattern is determined but uses a different arrangement of nuclease subunits. Further structural similarity to a broad group of metal/phosphate-binding proteins, including conservation of active-site location, is observed. A high degree of conservation of surface electrostatic character is observed between Hjc and T4-phage endonuclease VII despite a complete lack of structural homology. A model of the Hjc-Holliday junction complex is proposed, based on the available functional and structural data.
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===THE STRUCTURE OF HJC, A HOLLIDAY JUNCTION RESOLVING ENZYME FROM SULFOLOBUS SOLFATARICUS===
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Structure of Hjc, a Holliday junction resolvase, from Sulfolobus solfataricus.,Bond CS, Kvaratskhelia M, Richard D, White MF, Hunter WN Proc Natl Acad Sci U S A. 2001 May 8;98(10):5509-14. Epub 2001 May 1. PMID:11331763<ref>PMID:11331763</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1hh1" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_11331763}}, adds the Publication Abstract to the page
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*[[Resolvase 3D structures|Resolvase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 11331763 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_11331763}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1HH1 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HH1 OCA].
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[[Category: Saccharolobus solfataricus]]
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[[Category: Bond CS]]
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==Reference==
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[[Category: Hunter WN]]
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<ref group="xtra">PMID:11331763</ref><references group="xtra"/>
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[[Category: Kvaratskhelia M]]
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[[Category: Sulfolobus solfataricus]]
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[[Category: Richard D]]
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[[Category: Bond, C S.]]
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[[Category: White MF]]
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[[Category: Hunter, W N.]]
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[[Category: Kvaratskhelia, M.]]
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[[Category: Richard, D.]]
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[[Category: White, M F.]]
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[[Category: Archaea]]
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[[Category: Holliday junction resolvase]]
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[[Category: Homologous recombination]]
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[[Category: Nuclease domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 11:04:24 2009''
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Current revision

THE STRUCTURE OF HJC, A HOLLIDAY JUNCTION RESOLVING ENZYME FROM SULFOLOBUS SOLFATARICUS

PDB ID 1hh1

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