2cua
From Proteopedia
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(New page: 200px<br /><applet load="2cua" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cua, resolution 1.60Å" /> '''THE CUA DOMAIN OF CY...) |
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| - | [[Image:2cua.jpg|left|200px]]<br /><applet load="2cua" size="450" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="2cua, resolution 1.60Å" /> | ||
| - | '''THE CUA DOMAIN OF CYTOCHROME BA3 FROM THERMUS THERMOPHILUS'''<br /> | ||
| - | == | + | ==THE CUA DOMAIN OF CYTOCHROME BA3 FROM THERMUS THERMOPHILUS== |
| - | The structure of the CuA-containing, extracellular domain of Thermus | + | <StructureSection load='2cua' size='340' side='right'caption='[[2cua]], [[Resolution|resolution]] 1.60Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2cua]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CUA FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CUA:DINUCLEAR+COPPER+ION'>CUA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cua OCA], [https://pdbe.org/2cua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cua RCSB], [https://www.ebi.ac.uk/pdbsum/2cua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cua ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/COX2_THETH COX2_THETH] Subunits I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B). | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cu/2cua_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cua ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The structure of the CuA-containing, extracellular domain of Thermus thermophilus ba3-type cytochrome c oxidase has been determined to 1.6 A resolution using multiple X-ray wavelength anomalous dispersion (MAD). The Cu2S2 cluster forms a planar rhombus with a copper-copper distance of 2.51 +/- 0.03 A. X-ray absorption fine-structure (EXAFS) studies show that this distance is unchanged by crystallization. The CuA center is asymmetrical; one copper is tetrahedrally coordinated to two bridging cysteine thiolates, one histidine nitrogen and one methionine sulfur, while the other is trigonally coordinated by the two cysteine thiolates and a histidine nitrogen. Combined sequence-structure alignment of amino acid sequences reveals conserved interactions between cytochrome c oxidase subunits I and II. | ||
| - | + | The CuA domain of Thermus thermophilus ba3-type cytochrome c oxidase at 1.6 A resolution.,Williams PA, Blackburn NJ, Sanders D, Bellamy H, Stura EA, Fee JA, McRee DE Nat Struct Biol. 1999 Jun;6(6):509-16. PMID:10360350<ref>PMID:10360350</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2cua" style="background-color:#fffaf0;"></div> | |
| - | [[Category: | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
| - | [[Category: Bellamy | + | [[Category: Bellamy H]] |
| - | [[Category: Blackburn | + | [[Category: Blackburn NJ]] |
| - | [[Category: Fee | + | [[Category: Fee JA]] |
| - | [[Category: Mcree | + | [[Category: Mcree DE]] |
| - | [[Category: Sanders | + | [[Category: Sanders D]] |
| - | [[Category: Stura | + | [[Category: Stura EA]] |
| - | [[Category: Williams | + | [[Category: Williams PA]] |
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Current revision
THE CUA DOMAIN OF CYTOCHROME BA3 FROM THERMUS THERMOPHILUS
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