2cn2

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{{Seed}}
 
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[[Image:2cn2.png|left|200px]]
 
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==Crystal Structures of Clostridium thermocellum Xyloglucanase==
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The line below this paragraph, containing "STRUCTURE_2cn2", creates the "Structure Box" on the page.
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<StructureSection load='2cn2' size='340' side='right'caption='[[2cn2]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2cn2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_thermocellus Acetivibrio thermocellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CN2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CN2 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_2cn2| PDB=2cn2 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cn2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cn2 OCA], [https://pdbe.org/2cn2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cn2 RCSB], [https://www.ebi.ac.uk/pdbsum/2cn2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cn2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/XG74_ACETH XG74_ACETH] Hydrolyzes the glucosidic bonds of unbranched Glc residues in tamarind seed xyloglucan, producing XXXG, XLXG, XXLG and XLLG. Has low activity on carboxymethylcellulose, lichenan,hydroxyethylcellulose and glucuronoxylan, and no activity on xylan, polygalaturonic acid, wheat arabinoxylan, rhamnogalacturan, curdlan, laminarin, galactomannan, galactan, arabinan and pachyman or amorphous cellulose.<ref>PMID:16207921</ref> <ref>PMID:16772298</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cn/2cn2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cn2 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The enzymatic degradation of the plant cell wall is central both to the natural carbon cycle and, increasingly, to environmentally friendly routes to biomass conversion, including the production of biofuels. The plant cell wall is a complex composite of cellulose microfibrils embedded in diverse polysaccharides collectively termed hemicelluloses. Xyloglucan is one such polysaccharide whose hydrolysis is catalyzed by diverse xyloglucanases. Here we present the structure of the Clostridium thermocellum xyloglucanase Xgh74A in both apo and ligand-complexed forms. The structures, in combination with mutagenesis data on the catalytic residues and the kinetics and specificity of xyloglucan hydrolysis reveal a complex subsite specificity accommodating seventeen monosaccharide moieties of the multibranched substrate in an open substrate binding terrain.
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===CRYSTAL STRUCTURES OF CLOSTRIDIUM THERMOCELLUM XYLOGLUCANASE===
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Crystal structures of Clostridium thermocellum xyloglucanase, XGH74A, reveal the structural basis for xyloglucan recognition and degradation.,Martinez-Fleites C, Guerreiro CI, Baumann MJ, Taylor EJ, Prates JA, Ferreira LM, Fontes CM, Brumer H, Davies GJ J Biol Chem. 2006 Aug 25;281(34):24922-33. Epub 2006 Jun 13. PMID:16772298<ref>PMID:16772298</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2cn2" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16772298}}, adds the Publication Abstract to the page
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16772298 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16772298}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Acetivibrio thermocellus]]
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2CN2 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CN2 OCA].
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[[Category: Large Structures]]
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[[Category: Baumann MJ]]
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==Reference==
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[[Category: Brumer H]]
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<ref group="xtra">PMID:16772298</ref><references group="xtra"/>
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[[Category: Davies GJ]]
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[[Category: Clostridium thermocellum]]
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[[Category: Ferreira LMA]]
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[[Category: Xyloglucan-specific endo-beta-1,4-glucanase]]
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[[Category: Fontes CMGA]]
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[[Category: Baumann, M J.]]
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[[Category: Guerreiro CI]]
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[[Category: Brumer, H.]]
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[[Category: Martinez-Fleites C]]
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[[Category: Davies, G J.]]
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[[Category: Prates JAM]]
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[[Category: Ferreira, L M.A.]]
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[[Category: Taylor EJ]]
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[[Category: Fontes, C M.G A.]]
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[[Category: Guerreiro, C I.]]
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[[Category: Martinez-Fleites, C.]]
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[[Category: Prates, J A.M.]]
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[[Category: Taylor, E J.]]
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[[Category: Gh74 family]]
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[[Category: Glycosylhydrolase]]
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[[Category: Hydrolase]]
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[[Category: Xyloglucanase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 11:23:49 2009''
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Current revision

Crystal Structures of Clostridium thermocellum Xyloglucanase

PDB ID 2cn2

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