2cwm

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(New page: 200px<br /><applet load="2cwm" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cwm, resolution 1.95&Aring;" /> '''Native Crystal Struc...)
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[[Image:2cwm.gif|left|200px]]<br /><applet load="2cwm" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2cwm, resolution 1.95&Aring;" />
 
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'''Native Crystal Structure of NO releasing inductive lectin from seeds of the Canavalia maritima (ConM)'''<br />
 
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==Overview==
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==Native Crystal Structure of NO releasing inductive lectin from seeds of the Canavalia maritima (ConM)==
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Here, we report the crystallographic study of a lectin from Canavalia, maritima seeds (ConM) and its relaxant activity on vascular smooth muscle, to provide new insights into the understanding of structure/function, relationships of this class of proteins. ConM was crystallized and its, structure determined by standard molecular replacement techniques. The, amino acid residues, previously suggested incorrectly by manual, sequencing, have now been determined as I17, I53, S129, S134, G144, S164, P165, S187, V190, S169, T196, and S202. Analysis of the structure, indicated a dimer in the asymmetric unit, two metal binding sites per, monomer, and loops involved in the molecular oligomerization. These confer, 98% similarity between ConM and other previously described lectins, derived from Canavalia ensiformis and Canavalia brasiliensis. Our, functional data indicate that ConM exerts a concentration-dependent, relaxant action on isolated aortic rings that probably occurs via an, interaction with a specific lectin-binding site on the endothelium, resulting in a release of nitric oxide.
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<StructureSection load='2cwm' size='340' side='right'caption='[[2cwm]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2cwm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_rosea Canavalia rosea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CWM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CWM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cwm OCA], [https://pdbe.org/2cwm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cwm RCSB], [https://www.ebi.ac.uk/pdbsum/2cwm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cwm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CONA_CANRO CONA_CANRO] Glucose/D-mannose specific lectin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cw/2cwm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cwm ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2CWM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Canavalia_maritima Canavalia maritima] with CA and MN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CWM OCA].
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*[[Concanavalin 3D structures|Concanavalin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Native crystal structure of a nitric oxide-releasing lectin from the seeds of Canavalia maritima., Gadelha CA, Moreno FB, Santi-Gadelha T, Cajazeiras JB, Rocha BA, Assreuy AM, Lima Mota MR, Pinto NV, Passos Meireles AV, Borges JC, Freitas BT, Canduri F, Souza EP, Delatorre P, Criddle DN, de Azevedo WF Jr, Cavada BS, J Struct Biol. 2005 Dec;152(3):185-94. Epub 2005 Nov 14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16337811 16337811]
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[[Category: Canavalia rosea]]
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[[Category: Canavalia maritima]]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Assreuy AMS]]
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[[Category: Assreuy, A.M.S.]]
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[[Category: Cavada BS]]
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[[Category: Cavada, B.S.]]
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[[Category: Criddle DN]]
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[[Category: Criddle, D.N.]]
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[[Category: De Azevedo Jr WF]]
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[[Category: Delatorre, P.]]
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[[Category: Delatorre P]]
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[[Category: Gadelha, C.A.A.]]
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[[Category: Gadelha CAA]]
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[[Category: Jr., W.F.De.Azevedo.]]
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[[Category: Moreno FBMB]]
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[[Category: Moreno, F.B.M.B.]]
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[[Category: Rocha BAM]]
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[[Category: Rocha, B.A.M.]]
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[[Category: Santi-Gadelha T]]
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[[Category: Santi-Gadelha, T.]]
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[[Category: Souza EP]]
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[[Category: Souza, E.P.]]
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[[Category: CA]]
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[[Category: MN]]
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[[Category: canavalia maritima]]
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[[Category: lectin]]
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[[Category: x-ray diffraction]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 09:18:36 2007''
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Current revision

Native Crystal Structure of NO releasing inductive lectin from seeds of the Canavalia maritima (ConM)

PDB ID 2cwm

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