1sfy

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{{Seed}}
 
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[[Image:1sfy.png|left|200px]]
 
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==Crystal structure of recombinant Erythrina corallodandron Lectin==
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The line below this paragraph, containing "STRUCTURE_1sfy", creates the "Structure Box" on the page.
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<StructureSection load='1sfy' size='340' side='right'caption='[[1sfy]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1sfy]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Erythrina_corallodendron Erythrina corallodendron]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SFY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SFY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PRD_900004:beta-lactose'>PRD_900004</scene></td></tr>
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{{STRUCTURE_1sfy| PDB=1sfy | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sfy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sfy OCA], [https://pdbe.org/1sfy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sfy RCSB], [https://www.ebi.ac.uk/pdbsum/1sfy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sfy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LEC_ERYCO LEC_ERYCO] Galactose and N-acetyllactosamine specific lectin. Binds to the H-2 blood type determinant fucosyl-N-acetyllactosamine.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sf/1sfy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sfy ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The three-dimensional structure of the recombinant form of Erythrina corallodendron lectin, complexed with lactose, has been elucidated by X-ray crystallography at 2.55 A resolution. Comparison of this non-glycosylated structure with that of the native glycosylated lectin reveals that the tertiary and quaternary structures are identical in the two forms, with local changes observed at one of the glycosylation sites (Asn17). These changes take place in such a way that hydrogen bonds with the neighboring protein molecules in rECorL compensate those made by the glycan with the protein in ECorL. Contrary to an earlier report, this study demonstrates that the glycan attached to the lectin does not influence the oligomeric state of the lectin. Identical interactions between the lectin and the non-covalently bound lactose in the two forms indicate, in line with earlier reports, that glycosylation does not affect the carbohydrate specificity of the lectin. The present study, the first of its kind involving a glycosylated protein with a well-defined glycan and the corresponding deglycosylated form, provides insights into the structural aspects of protein glycosylation.
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===Crystal structure of recombinant Erythrina corallodandron Lectin===
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Effect of glycosylation on the structure of Erythrina corallodendron lectin.,Kulkarni KA, Srivastava A, Mitra N, Sharon N, Surolia A, Vijayan M, Suguna K Proteins. 2004 Sep 1;56(4):821-7. PMID:15281133<ref>PMID:15281133</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15281133}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1sfy" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15281133 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15281133}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1SFY is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Erythrina_corallodendron Erythrina corallodendron]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SFY OCA].
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==Reference==
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<ref group="xtra">PMID:15281133</ref><references group="xtra"/>
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[[Category: Erythrina corallodendron]]
[[Category: Erythrina corallodendron]]
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[[Category: Kulkarni, K A.]]
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[[Category: Large Structures]]
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[[Category: Mitra, N.]]
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[[Category: Kulkarni KA]]
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[[Category: Srivastava, A.]]
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[[Category: Mitra N]]
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[[Category: Suguna, K.]]
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[[Category: Srivastava A]]
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[[Category: Surolia, A.]]
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[[Category: Suguna K]]
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[[Category: Vijayan, M.]]
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[[Category: Surolia A]]
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[[Category: Erythrina lectin]]
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[[Category: Vijayan M]]
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[[Category: Glycosylation]]
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[[Category: Legume lectin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 11:36:46 2009''
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Current revision

Crystal structure of recombinant Erythrina corallodandron Lectin

PDB ID 1sfy

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