2pjr

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{{Seed}}
 
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[[Image:2pjr.png|left|200px]]
 
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==HELICASE PRODUCT COMPLEX==
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The line below this paragraph, containing "STRUCTURE_2pjr", creates the "Structure Box" on the page.
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<StructureSection load='2pjr' size='340' side='right'caption='[[2pjr]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2pjr]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PJR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PJR FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2pjr| PDB=2pjr | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pjr OCA], [https://pdbe.org/2pjr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pjr RCSB], [https://www.ebi.ac.uk/pdbsum/2pjr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pjr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PCRA_GEOSE PCRA_GEOSE] DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substrate with a 5' tail. Preferred substrate being one with both single and double-stranded regions of DNA.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pj/2pjr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pjr ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We have determined two different structures of PcrA DNA helicase complexed with the same single strand tailed DNA duplex, providing snapshots of different steps on the catalytic pathway. One of the structures is of a complex with a nonhydrolyzable analog of ATP and is thus a "substrate" complex. The other structure contains a bound sulphate ion that sits in a position equivalent to that occupied by the phosphate ion produced after ATP hydrolysis, thereby mimicking a "product" complex. In both complexes, the protein is monomeric. Large and distinct conformational changes occur on binding DNA and the nucleotide cofactor. Taken together, these structures provide evidence against an "active rolling" model for helicase action but are instead consistent with an "inchworm" mechanism.
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===HELICASE PRODUCT COMPLEX===
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Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism.,Velankar SS, Soultanas P, Dillingham MS, Subramanya HS, Wigley DB Cell. 1999 Apr 2;97(1):75-84. PMID:10199404<ref>PMID:10199404</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2pjr" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_10199404}}, adds the Publication Abstract to the page
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*[[Helicase|Helicase]]
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(as it appears on PubMed at http://www.pubmed.gov), where 10199404 is the PubMed ID number.
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*[[Helicase 3D structures|Helicase 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_10199404}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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2PJR is a 8 chains structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PJR OCA].
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==Reference==
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<ref group="xtra">PMID:10199404</ref><references group="xtra"/>
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[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
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[[Category: Dillingham, M S.]]
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[[Category: Large Structures]]
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[[Category: Soultanas, P.]]
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[[Category: Dillingham MS]]
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[[Category: Subramanya, H S.]]
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[[Category: Soultanas P]]
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[[Category: Velankar, S S.]]
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[[Category: Subramanya HS]]
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[[Category: Wigley, D B.]]
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[[Category: Velankar SS]]
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[[Category: Dna]]
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[[Category: Wigley DB]]
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[[Category: Helicase pcra]]
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[[Category: Hydrolase]]
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[[Category: Product complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 11:39:00 2009''
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Current revision

HELICASE PRODUCT COMPLEX

PDB ID 2pjr

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