1xlm

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:39, 9 August 2023) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:1xlm.png|left|200px]]
 
-
<!--
+
==D254E, D256E MUTANT OF D-XYLOSE ISOMERASE COMPLEXED WITH AL3 AND XYLITOL==
-
The line below this paragraph, containing "STRUCTURE_1xlm", creates the "Structure Box" on the page.
+
<StructureSection load='1xlm' size='340' side='right'caption='[[1xlm]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1xlm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arthrobacter_sp._NRRL_B3728 Arthrobacter sp. NRRL B3728]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XLM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XLM FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AL:ALUMINUM+ION'>AL</scene>, <scene name='pdbligand=XYL:D-XYLITOL'>XYL</scene></td></tr>
-
{{STRUCTURE_1xlm| PDB=1xlm | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xlm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xlm OCA], [https://pdbe.org/1xlm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xlm RCSB], [https://www.ebi.ac.uk/pdbsum/1xlm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xlm ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/XYLA_ARTS7 XYLA_ARTS7]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xl/1xlm_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xlm ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The structure of the D254.256E double mutant of Arthrobacter xylose isomerase with Al3+ at both metal-binding sites was determined by the molecular replacement method at a conventional R-factor of 0.179. Binding of the two Al3+ does not alter the overall structure significantly. However, there are local rearrangements in the octahedral co-ordination sphere of the Al3+. The inhibitor molecule moves somewhat away from the active site. Furthermore, evidence was revealed for metal ion movement from site 2(1) to site 2(2) upon double mutation. Xylose isomerase requires two divalent metal cations for activation. The catalytic metal ion is translocated 1.8 A away from its initial position during the catalytic reaction. The fact that both activating and inactivating metals (including Al3+) were found exclusively at a single location in the double mutant was an indication that the consequently missing shuttle may account for the crippled catalytic efficiency.
-
===D254E, D256E MUTANT OF D-XYLOSE ISOMERASE COMPLEXED WITH AL3 AND XYLITOL===
+
Structure determination and refinement of the Al3+ complex of the D254,256E mutant of Arthrobacter D-xylose isomerase at 2.40 A resolution. Further evidence for inhibitor-induced metal ion movement.,Gerczei T, Bocskei Z, Szabo E, Asboth B, Naray-Szabo G Int J Biol Macromol. 1999 Aug;25(4):329-36. PMID:10456773<ref>PMID:10456773</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 1xlm" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_2319597}}, adds the Publication Abstract to the page
+
*[[D-xylose isomerase 3D structures|D-xylose isomerase 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 2319597 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_2319597}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Arthrobacter sp. NRRL B3728]]
-
1XLM is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Arthrobacter_sp. Arthrobacter sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XLM OCA].
+
[[Category: Large Structures]]
-
 
+
[[Category: Asboth B]]
-
==Reference==
+
[[Category: Bocskei ZS]]
-
<ref group="xtra">PMID:2319597</ref><references group="xtra"/>
+
[[Category: Gerczei T]]
-
[[Category: Arthrobacter sp.]]
+
[[Category: Naray-Szabo G]]
-
[[Category: Xylose isomerase]]
+
[[Category: Szabo E]]
-
[[Category: Asboth, B.]]
+
-
[[Category: Bocskei, Z S.]]
+
-
[[Category: Gerczei, T.]]
+
-
[[Category: Naray-Szabo, G.]]
+
-
[[Category: Szabo, E.]]
+
-
[[Category: Al]]
+
-
[[Category: Isomerase]]
+
-
[[Category: Pentose shunt]]
+
-
[[Category: Substrate induced metal ion movement]]
+
-
[[Category: Xylose metabolism]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 11:59:16 2009''
+

Current revision

D254E, D256E MUTANT OF D-XYLOSE ISOMERASE COMPLEXED WITH AL3 AND XYLITOL

PDB ID 1xlm

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xlm"
Personal tools