2pan

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{{Seed}}
 
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[[Image:2pan.png|left|200px]]
 
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==Crystal structure of E. coli glyoxylate carboligase==
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The line below this paragraph, containing "STRUCTURE_2pan", creates the "Structure Box" on the page.
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<StructureSection load='2pan' size='340' side='right'caption='[[2pan]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2pan]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PAN FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
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-->
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_2pan| PDB=2pan | SCENE= }}
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gcl ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Tartronate-semialdehyde_synthase Tartronate-semialdehyde synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.47 4.1.1.47] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pan OCA], [https://pdbe.org/2pan PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pan RCSB], [https://www.ebi.ac.uk/pdbsum/2pan PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pan ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[https://www.uniprot.org/uniprot/GCL_ECOLI GCL_ECOLI]] Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pa/2pan_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pan ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Thiamine diphosphate (ThDP), a derivative of vitamin B1, is an enzymatic cofactor whose special chemical properties allow it to play critical mechanistic roles in a number of essential metabolic enzymes. It has been assumed that all ThDP-dependent enzymes exploit a polar interaction between a strictly conserved glutamate and the N1' of the ThDP moiety. The crystal structure of glyoxylate carboligase challenges this paradigm by revealing that valine replaces the conserved glutamate. Through kinetic, spectroscopic and site-directed mutagenesis studies, we show that although this extreme change lowers the rate of the initial step of the enzymatic reaction, it ensures efficient progress through subsequent steps. Glyoxylate carboligase thus provides a unique illustration of the fine tuning between catalytic stages imposed during evolution on enzymes catalyzing multistep processes.
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===Crystal structure of E. coli glyoxylate carboligase===
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Glyoxylate carboligase lacks the canonical active site glutamate of thiamine-dependent enzymes.,Kaplun A, Binshtein E, Vyazmensky M, Steinmetz A, Barak Z, Chipman DM, Tittmann K, Shaanan B Nat Chem Biol. 2008 Feb;4(2):113-8. Epub 2008 Jan 6. PMID:18176558<ref>PMID:18176558</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18176558}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2pan" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18176558 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18176558}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Bacillus coli migula 1895]]
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2PAN is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PAN OCA].
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[[Category: Large Structures]]
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==Reference==
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<ref group="xtra">PMID:18176558</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
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[[Category: Tartronate-semialdehyde synthase]]
[[Category: Tartronate-semialdehyde synthase]]
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[[Category: Barak, Z.]]
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[[Category: Barak, Z]]
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[[Category: Chipman, D M.]]
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[[Category: Chipman, D M]]
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[[Category: Kaplun, A.]]
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[[Category: Kaplun, A]]
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[[Category: Shaanan, B.]]
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[[Category: Shaanan, B]]
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[[Category: Vyazmensky, M.]]
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[[Category: Vyazmensky, M]]
[[Category: Enzyme]]
[[Category: Enzyme]]
[[Category: Fad]]
[[Category: Fad]]
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[[Category: Lyase]]
[[Category: Lyase]]
[[Category: Thimain-dependent enzyme]]
[[Category: Thimain-dependent enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 12:02:14 2009''
 

Current revision

Crystal structure of E. coli glyoxylate carboligase

PDB ID 2pan

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