2jml

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{{Seed}}
 
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[[Image:2jml.png|left|200px]]
 
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==Solution structure of the N-terminal domain of CarA repressor==
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The line below this paragraph, containing "STRUCTURE_2jml", creates the "Structure Box" on the page.
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<StructureSection load='2jml' size='340' side='right'caption='[[2jml]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2jml]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Myxococcus_xanthus_DK_1622 Myxococcus xanthus DK 1622]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JML FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jml OCA], [https://pdbe.org/2jml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jml RCSB], [https://www.ebi.ac.uk/pdbsum/2jml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jml ProSAT]</span></td></tr>
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{{STRUCTURE_2jml| PDB=2jml | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q1DDV9_MYXXD Q1DDV9_MYXXD]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jm/2jml_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jml ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Blue light induces carotenogenesis in Myxococcus xanthus. The carB operon encodes all but one of the structural genes involved, and its expression is regulated by the CarA-CarS repressor-antirepressor pair. In the dark, CarA-operator binding represses carB. CarS, produced on illumination, interacts physically with CarA to dismantle the CarA-operator complex and activate carB. Both operator and CarS bind to the autonomously folded N-terminal domain of CarA, CarA(Nter), which in excess represses carB. Here, we report the NMR structure of CarA(Nter), and map residues that interact with operator and CarS by NMR chemical shift perturbations, and in vivo and in vitro analyses of site-directed mutants. We show CarA(Nter) adopts the winged-helix topology of MerR-family DNA-binding domains, and conserves the majority of the helix-turn-helix and wing contacts with DNA. Tellingly, helix alpha2 in CarA, a key element in operator DNA recognition, is also critical for interaction with CarS, implying that the CarA-CarS protein-protein and the CarA-operator protein-DNA interfaces overlap. Thus, binding of CarA to operator and to antirepressor are mutually exclusive, and CarA may discern structural features in the acidic CarS protein that resemble operator DNA. Repressor inactivation by occluding the DNA-binding region may be a recurrent mechanism of action for acidic antirepressors.
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===Solution structure of the N-terminal domain of CarA repressor===
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Structural basis for operator and antirepressor recognition by Myxococcus xanthus CarA repressor.,Navarro-Aviles G, Jimenez MA, Perez-Marin MC, Gonzalez C, Rico M, Murillo FJ, Elias-Arnanz M, Padmanabhan S Mol Microbiol. 2007 Feb;63(4):980-94. PMID:17233828<ref>PMID:17233828</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<!--
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17233828}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2jml" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17233828 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17233828}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2JML is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Myxococcus_xanthus Myxococcus xanthus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JML OCA].
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[[Category: Myxococcus xanthus DK 1622]]
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[[Category: Elias-Arnanz M]]
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==Reference==
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[[Category: Gonzalez C]]
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<ref group="xtra">PMID:17233828</ref><references group="xtra"/>
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[[Category: Jimenez M]]
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[[Category: Myxococcus xanthus]]
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[[Category: Murillo FJ]]
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[[Category: Elias-Arnanz, M.]]
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[[Category: Padmanabhan S]]
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[[Category: Gonzalez, C.]]
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[[Category: Perez-Marin MC]]
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[[Category: Jimenez, M.]]
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[[Category: Rico M]]
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[[Category: Murillo, F J.]]
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[[Category: Padmanabhan, S.]]
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[[Category: Perez-Marin, M C.]]
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[[Category: Rico, M.]]
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[[Category: Anti-repressor]]
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[[Category: Carotenogenesis]]
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[[Category: Merr]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 12:05:56 2009''
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Current revision

Solution structure of the N-terminal domain of CarA repressor

PDB ID 2jml

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