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2d1u

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(New page: 200px<br /><applet load="2d1u" size="450" color="white" frame="true" align="right" spinBox="true" caption="2d1u" /> '''Solution strcuture of the periplasmic signal...)
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[[Image:2d1u.gif|left|200px]]<br /><applet load="2d1u" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2d1u" />
 
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'''Solution strcuture of the periplasmic signaling domain of FecA from Escherichia coli'''<br />
 
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==Overview==
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==Solution structure of the periplasmic signaling domain of FecA from Escherichia coli==
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Gram-negative bacteria possess outer membrane receptors that utilize, energy provided by the TonB system to take up iron. Several of these, receptors participate in extracytoplasmic factor (ECF) signalling through, an N-terminal signalling domain that interacts with a periplasmic, transmembrane anti-sigma factor protein and a cytoplasmic sigma factor, protein. The structures of the intact TonB-dependent outer membrane, receptor FecA from Escherichia coli and FpvA from Pseudomonas aeruginosa, have recently been solved by protein crystallography; however, no electron, density was detected for their periplasmic signalling domains, suggesting, that it was either unfolded or flexible with respect to the remainder of, the protein. Here we describe the well-defined solution structure of this, domain solved by multidimensional nuclear magnetic resonance (NMR), spectroscopy. The monomeric protein construct contains the 79-residue, N-terminal domain as well as the next 17 residues that are part of the, receptor's plug domain. These form two clearly distinct regions: a highly, structured domain at the N-terminal end followed by an extended flexible, tail at the C-terminal end, which includes the 'TonB-box' region, and, connects it to the plug domain of the receptor. The structured region, consists of two alpha-helices that are positioned side by side and are, sandwiched in between two small beta-sheets. This is a novel protein fold, which appears to be preserved in all the periplasmic signalling domains of, bacterial TonB-dependent outer membrane receptors that are involved in ECF, signalling, because the hydrophobic residues that make up the core of the, protein domain are highly conserved.
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<StructureSection load='2d1u' size='340' side='right'caption='[[2d1u]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2d1u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D1U FirstGlance]. <br>
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2D1U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d1u OCA], [https://pdbe.org/2d1u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d1u RCSB], [https://www.ebi.ac.uk/pdbsum/2d1u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d1u ProSAT]</span></td></tr>
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==Reference==
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</table>
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Nuclear magnetic resonance solution structure of the periplasmic signalling domain of the TonB-dependent outer membrane transporter FecA from Escherichia coli., Garcia-Herrero A, Vogel HJ, Mol Microbiol. 2005 Dec;58(5):1226-37. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16313612 16313612]
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== Function ==
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[https://www.uniprot.org/uniprot/FECA_ECOLI FECA_ECOLI] FecA is the outer membrane receptor protein in the Fe(3+) dicitrate transport system.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d1/2d1u_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d1u ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Garcia-Herrero, A.]]
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[[Category: Garcia-Herrero A]]
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[[Category: Vogel, H.J.]]
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[[Category: Vogel HJ]]
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[[Category: feca]]
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[[Category: iron-uptake]]
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[[Category: surface signaling]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 09:23:39 2007''
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Current revision

Solution structure of the periplasmic signaling domain of FecA from Escherichia coli

PDB ID 2d1u

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