2d33

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(New page: 200px<br /><applet load="2d33" size="450" color="white" frame="true" align="right" spinBox="true" caption="2d33, resolution 2.60&Aring;" /> '''Crystal Structure of...)
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[[Image:2d33.gif|left|200px]]<br /><applet load="2d33" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2d33, resolution 2.60&Aring;" />
 
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'''Crystal Structure of gamma-Glutamylcysteine Synthetase Complexed with Aluminum Fluoride'''<br />
 
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==Overview==
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==Crystal Structure of gamma-Glutamylcysteine Synthetase Complexed with Aluminum Fluoride==
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Gamma-glutamylcysteine synthetase (gammaGCS), a rate-limiting enzyme in, glutathione biosynthesis, plays a central role in glutathione homeostasis, and is a target for development of potential therapeutic agents against, parasites and cancer. We have determined the crystal structures of, Escherichia coli gammaGCS unliganded and complexed with a, sulfoximine-based transition-state analog inhibitor at resolutions of 2.5, and 2.1 A, respectively. In the crystal structure of the complex, the, bound inhibitor is phosphorylated at the sulfoximido nitrogen and is, coordinated to three Mg2+ ions. The cysteine-binding site was identified;, it is formed inductively at the transition state. In the unliganded, structure, an open space exists around the representative cysteine-binding, site and is probably responsible for the competitive binding of, glutathione. Upon inhibitor binding, the side chains of Tyr-241 and, Tyr-300 turn, forming a hydrogen-bonding triad with the carboxyl group of, the inhibitor's cysteine moiety, allowing this moiety to fit tightly into, the cysteine-binding site with concomitant accommodation of its side chain, into a shallow pocket. This movement is caused by a conformational change, of a switch loop (residues 240-249). Based on this crystal structure, the, cysteine-binding sites of mammalian and parasitic gammaGCSs were predicted, by multiple sequence alignment, although no significant sequence identity, exists between the E. coli gammaGCS and its eukaryotic homologues. The, identification of this cysteine-binding site provides important, information for the rational design of novel gammaGCS inhibitors.
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<StructureSection load='2d33' size='340' side='right'caption='[[2d33]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2d33]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D33 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D33 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=CYS:CYSTEINE'>CYS</scene>, <scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d33 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d33 OCA], [https://pdbe.org/2d33 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d33 RCSB], [https://www.ebi.ac.uk/pdbsum/2d33 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d33 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GSH1_ECOLI GSH1_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d3/2d33_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d33 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Escherichia coli B gamma-glutamylcysteine synthetase (gammaGCS) catalyzes the ATP-dependent coupling of L-Glu and L-Cys to form the glutathione precursor gamma-L-Glu-Cys and is a target for development of potential therapeutic agents. By introducing four point mutations of surface-exposed cysteine residues to serine, the gammaGCS was purified to homogeneity; single crystals have been obtained using the hanging-drop vapour-diffusion method with sodium formate. The gammaGCS crystal diffracted to 2.8 A and belongs to space group R3, with unit-cell parameters a = b = 326.7, c = 103.9 A.
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==About this Structure==
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Escherichia coli B gamma-glutamylcysteine synthetase: modification, purification, crystallization and preliminary crystallographic analysis.,Hibi T, Hisada H, Nakatsu T, Kato H, Oda J Acta Crystallogr D Biol Crystallogr. 2002 Feb;58(Pt 2):316-8. Epub 2002, Jan 24. PMID:011807262<ref>PMID:011807262</ref>
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2D33 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG, GLU, CYS, ADP and AF3 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glutamate--cysteine_ligase Glutamate--cysteine ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.2 6.3.2.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2D33 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of gamma-glutamylcysteine synthetase: insights into the mechanism of catalysis by a key enzyme for glutathione homeostasis., Hibi T, Nii H, Nakatsu T, Kimura A, Kato H, Hiratake J, Oda J, Proc Natl Acad Sci U S A. 2004 Oct 19;101(42):15052-7. Epub 2004 Oct 11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15477603 15477603]
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</div>
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<div class="pdbe-citations 2d33" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Glutamate--cysteine ligase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Hibi T]]
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[[Category: Hibi, T.]]
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[[Category: Katano H]]
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[[Category: Katano, H.]]
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[[Category: Kurokawa Y]]
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[[Category: Kurokawa, Y.]]
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[[Category: Nakayama M]]
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[[Category: Nakayama, M.]]
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[[Category: Nii H]]
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[[Category: Nii, H.]]
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[[Category: Oda J]]
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[[Category: Oda, J.]]
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[[Category: ADP]]
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[[Category: AF3]]
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[[Category: CYS]]
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[[Category: GLU]]
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[[Category: MG]]
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[[Category: glutathione homeostasis]]
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[[Category: peptide synthesis]]
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[[Category: phosphoryl transfer]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 09:24:52 2007''
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Current revision

Crystal Structure of gamma-Glutamylcysteine Synthetase Complexed with Aluminum Fluoride

PDB ID 2d33

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