2ido

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{{Seed}}
 
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[[Image:2ido.png|left|200px]]
 
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==Structure of the E. coli Pol III epsilon-Hot proofreading complex==
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The line below this paragraph, containing "STRUCTURE_2ido", creates the "Structure Box" on the page.
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<StructureSection load='2ido' size='340' side='right'caption='[[2ido]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2ido]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_virus_P1 Escherichia virus P1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IDO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IDO FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=TMP:THYMIDINE-5-PHOSPHATE'>TMP</scene></td></tr>
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{{STRUCTURE_2ido| PDB=2ido | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ido FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ido OCA], [https://pdbe.org/2ido PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ido RCSB], [https://www.ebi.ac.uk/pdbsum/2ido PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ido ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DPO3E_ECOLI DPO3E_ECOLI] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/id/2ido_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ido ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The epsilon subunit of Escherichia coli DNA polymerase III possesses 3'-exonucleolytic proofreading activity. Within the Pol III core, epsilon is tightly bound between the alpha subunit (DNA polymerase) and subunit. Here, we present the crystal structure of epsilon in complex with HOT, the bacteriophage P1-encoded homolog of , at 2.1 A resolution. The epsilon-HOT interface is defined by two areas of contact: an interaction of the previously unstructured N terminus of HOT with an edge of the epsilon central beta-sheet as well as interactions between HOT and the catalytically important helix alpha1-loop-helix alpha2 motif of epsilon. This structure provides insight into how HOT and, by implication, may stabilize the epsilon subunit, thus promoting efficient proofreading during chromosomal replication.
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===Structure of the E. coli Pol III epsilon-Hot proofreading complex===
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Structure of the Escherichia coli DNA polymerase III epsilon-HOT proofreading complex.,Kirby TW, Harvey S, DeRose EF, Chalov S, Chikova AK, Perrino FW, Schaaper RM, London RE, Pedersen LC J Biol Chem. 2006 Dec 15;281(50):38466-71. Epub 2006 Sep 13. PMID:16973612<ref>PMID:16973612</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2ido" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16973612}}, adds the Publication Abstract to the page
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*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16973612 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16973612}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2IDO is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p1 Enterobacteria phage p1] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IDO OCA].
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==Reference==
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<ref group="xtra">PMID:16973612</ref><references group="xtra"/>
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[[Category: DNA-directed DNA polymerase]]
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[[Category: Enterobacteria phage p1]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Chalov, S.]]
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[[Category: Escherichia virus P1]]
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[[Category: Chikova, A K.]]
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[[Category: Large Structures]]
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[[Category: DeRose, E F.]]
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[[Category: Chalov S]]
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[[Category: Harvey, S.]]
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[[Category: Chikova AK]]
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[[Category: Kirby, T W.]]
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[[Category: DeRose EF]]
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[[Category: London, R E.]]
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[[Category: Harvey S]]
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[[Category: Pedersen, L C.]]
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[[Category: Kirby TW]]
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[[Category: Perrino, F W.]]
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[[Category: London RE]]
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[[Category: Schaaper, R M.]]
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[[Category: Pedersen LC]]
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[[Category: Epsilon]]
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[[Category: Perrino FW]]
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[[Category: Exonuclease]]
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[[Category: Schaaper RM]]
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[[Category: Hot]]
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[[Category: Pol iii]]
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[[Category: Polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 12:58:23 2009''
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Current revision

Structure of the E. coli Pol III epsilon-Hot proofreading complex

PDB ID 2ido

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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