1lqc

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{{Seed}}
 
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[[Image:1lqc.png|left|200px]]
 
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==LAC REPRESSOR HEADPIECE (RESIDUES 1-56), NMR, 32 STRUCTURES==
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The line below this paragraph, containing "STRUCTURE_1lqc", creates the "Structure Box" on the page.
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<StructureSection load='1lqc' size='340' side='right'caption='[[1lqc]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1lqc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LQC FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lqc OCA], [https://pdbe.org/1lqc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lqc RCSB], [https://www.ebi.ac.uk/pdbsum/1lqc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lqc ProSAT]</span></td></tr>
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{{STRUCTURE_1lqc| PDB=1lqc | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LACI_ECOLI LACI_ECOLI] Repressor of the lactose operon. Binds allolactose as an inducer.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lq/1lqc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lqc ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The solution structure of the DNA binding domain of lac repressor (headpiece 1-56; HP56) has been refined using data from 2D and 3D NMR spectroscopy. The structure was derived from 1546 restraints (giving an average of 27.6 per residue), comprising 389 intraresidual, 402 sequential, 385 medium range and 325 long range distance restraints and also 30 phi and 15 chi 1 dihedral angle restraints. The structures were determined by the method of direct refinement against nuclear Overhauser enhancement peak volumes with the program DINOSAUR. The final set of 32 selected structures displayed an r.m.s. deviation from the average of 0.43(+/-0.08) A angstroms (backbone) and 0.95(+/-0.08) angstroms (all heavy atoms) for the best defined region of the protein (residues 3 to 49). The ensemble R-factor was 0.35, which indicates close correspondence with the experimental data. The structures revealed good stereochemical qualities. The conformations of the NMR structures of free and DNA complexed lac repressor headpiece were compared. The regions comprising the secondary structure elements show close correspondence for both conformations. However, the conformation of the loop between helix II and III changes considerably upon complexation of the headpiece. This change in the conformation of the loop in lac HP56 is essential for binding of the side-chains of residues Asn25 and His29 to the lac operator DNA. Finally, the lac headpiece residues that are intolerant to mutations were analysed. Most of these mutation-sensitive residues are important for a correct folding of the headpiece region, and a number of these residues are also involved in contacting the operator DNA.
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===LAC REPRESSOR HEADPIECE (RESIDUES 1-56), NMR, 32 STRUCTURES===
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Refined structure of lac repressor headpiece (1-56) determined by relaxation matrix calculations from 2D and 3D NOE data: change of tertiary structure upon binding to the lac operator.,Slijper M, Bonvin AM, Boelens R, Kaptein R J Mol Biol. 1996 Jun 21;259(4):761-73. PMID:8683581<ref>PMID:8683581</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1lqc" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_8683581}}, adds the Publication Abstract to the page
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*[[Lac repressor|Lac repressor]]
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(as it appears on PubMed at http://www.pubmed.gov), where 8683581 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_8683581}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1LQC is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LQC OCA].
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==Reference==
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<ref group="xtra">PMID:8683581</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Boelens, R.]]
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[[Category: Large Structures]]
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[[Category: Bonvin, A M.J J.]]
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[[Category: Boelens R]]
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[[Category: Kaptein, R.]]
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[[Category: Bonvin AMJJ]]
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[[Category: Slijper, M.]]
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[[Category: Kaptein R]]
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[[Category: Dna]]
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[[Category: Slijper M]]
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[[Category: Headpiece]]
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[[Category: Lac operon]]
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[[Category: Lac repressor]]
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[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 13:03:47 2009''
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Current revision

LAC REPRESSOR HEADPIECE (RESIDUES 1-56), NMR, 32 STRUCTURES

PDB ID 1lqc

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