2gft

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{{Seed}}
 
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[[Image:2gft.png|left|200px]]
 
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==Crystal structure of the E263A nucleophile mutant of Bacillus licheniformis endo-beta-1,4-galactanase in complex with galactotriose==
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The line below this paragraph, containing "STRUCTURE_2gft", creates the "Structure Box" on the page.
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<StructureSection load='2gft' size='340' side='right'caption='[[2gft]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2gft]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GFT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GFT FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=PRD_900114:4beta-beta-galactotriose'>PRD_900114</scene></td></tr>
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{{STRUCTURE_2gft| PDB=2gft | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gft OCA], [https://pdbe.org/2gft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gft RCSB], [https://www.ebi.ac.uk/pdbsum/2gft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gft ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GANB_BACLD GANB_BACLD] Involved in galactan degradation (PubMed:15312766). Degrades arabinose-free galactan to galactooligosaccharides, producing galactotetraose as the main product along with galactotriose, galactobiose, and galactose (PubMed:15312766). May hydrolyze the beta-1,4-galactan linkages of the galactan portion of arabinogalactan type I, a pectic plant polysaccharide from which most of the arabinose has been removed (By similarity).[UniProtKB:O07013]<ref>PMID:15312766</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gf/2gft_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gft ConSurf].
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<div style="clear:both"></div>
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===Crystal structure of the E263A nucleophile mutant of Bacillus licheniformis endo-beta-1,4-galactanase in complex with galactotriose===
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==See Also==
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*[[Beta-1%2C4-galactanase|Beta-1%2C4-galactanase]]
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== References ==
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==About this Structure==
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<references/>
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2GFT is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GFT OCA].
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__TOC__
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:15312766</ref><references group="xtra"/>
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[[Category: Arabinogalactan endo-1,4-beta-galactosidase]]
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[[Category: Bacillus licheniformis]]
[[Category: Bacillus licheniformis]]
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[[Category: Christensen, L L.H.]]
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[[Category: Large Structures]]
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[[Category: Jorgensen, C T.]]
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[[Category: Christensen LLH]]
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[[Category: Larsen, S.]]
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[[Category: De Maria L]]
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[[Category: Leggio, L Lo.]]
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[[Category: Jorgensen CT]]
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[[Category: Maria, L De.]]
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[[Category: Larsen S]]
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[[Category: Nours, J Le.]]
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[[Category: Le Nours J]]
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[[Category: Welner, D]]
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[[Category: Lo Leggio L]]
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[[Category: Beta-alpha-8-barrel]]
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[[Category: Welner D]]
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[[Category: Nucleophile inactive mutant]]
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[[Category: Protein-oligosaccharide complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 13:38:12 2009''
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Current revision

Crystal structure of the E263A nucleophile mutant of Bacillus licheniformis endo-beta-1,4-galactanase in complex with galactotriose

PDB ID 2gft

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