2z6y

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{{Seed}}
 
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[[Image:2z6y.png|left|200px]]
 
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==Crystal structure of a photoswitchable GFP-like protein Dronpa in the bright-state==
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The line below this paragraph, containing "STRUCTURE_2z6y", creates the "Structure Box" on the page.
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<StructureSection load='2z6y' size='340' side='right'caption='[[2z6y]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2z6y]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Echinophyllia_sp._SC22 Echinophyllia sp. SC22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z6Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z6Y FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr>
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{{STRUCTURE_2z6y| PDB=2z6y | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z6y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z6y OCA], [https://pdbe.org/2z6y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z6y RCSB], [https://www.ebi.ac.uk/pdbsum/2z6y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z6y ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q5TLG6_9CNID Q5TLG6_9CNID]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z6/2z6y_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z6y ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structural basis for the photochromism in the fluorescent protein Dronpa is poorly understood, because the crystal structures of the bright state of the protein did not provide an answer to the mechanism of the photochromism, and structural determination of the dark state has been elusive. We performed NMR analyses of Dronpa in solution at ambient temperatures to find structural flexibility of the protein in the dark state. Light-induced changes in interactions between the chromophore and beta-barrel are responsible for switching between the two states. In the bright state, the apex of the chromophore tethers to the barrel by a hydrogen bond, and an imidazole ring protruding from the barrel stabilizes the plane of the chromophore. These interactions are disrupted by strong illumination with blue light, and the chromophore, together with a part of the beta-barrel, becomes flexible, leading to a nonradiative decay process.
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===Crystal structure of a photoswitchable GFP-like protein Dronpa in the bright-state===
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Light-dependent regulation of structural flexibility in a photochromic fluorescent protein.,Mizuno H, Mal TK, Walchli M, Kikuchi A, Fukano T, Ando R, Jeyakanthan J, Taka J, Shiro Y, Ikura M, Miyawaki A Proc Natl Acad Sci U S A. 2008 Jul 8;105(27):9227-32. Epub 2008 Jun 23. PMID:18574155<ref>PMID:18574155</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2z6y" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18574155}}, adds the Publication Abstract to the page
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*[[Dronpa|Dronpa]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18574155 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18574155}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Echinophyllia sp. SC22]]
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2Z6Y is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Echinophyllia_sp._sc22 Echinophyllia sp. sc22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z6Y OCA].
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[[Category: Large Structures]]
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[[Category: Jeyakanthan J]]
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==Reference==
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[[Category: Kikuchi A]]
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<ref group="xtra">PMID:18574155</ref><references group="xtra"/>
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[[Category: Miyawaki A]]
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[[Category: Echinophyllia sp. sc22]]
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[[Category: Mizuno H]]
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[[Category: Jeyakanthan, J.]]
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[[Category: Shiro Y]]
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[[Category: Kikuchi, A.]]
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[[Category: Taka J]]
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[[Category: Miyawaki, A.]]
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[[Category: Mizuno, H.]]
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[[Category: Shiro, Y.]]
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[[Category: Taka, J.]]
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[[Category: Fluorescent protein]]
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[[Category: Gfp-like protein]]
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[[Category: Photochromism]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 13:56:05 2009''
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Current revision

Crystal structure of a photoswitchable GFP-like protein Dronpa in the bright-state

PDB ID 2z6y

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