1yn9

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{{Seed}}
 
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[[Image:1yn9.png|left|200px]]
 
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==Crystal structure of baculovirus RNA 5'-phosphatase complexed with phosphate==
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The line below this paragraph, containing "STRUCTURE_1yn9", creates the "Structure Box" on the page.
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<StructureSection load='1yn9' size='340' side='right'caption='[[1yn9]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1yn9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Autographa_californica_nucleopolyhedrovirus Autographa californica nucleopolyhedrovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YN9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YN9 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_1yn9| PDB=1yn9 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yn9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yn9 OCA], [https://pdbe.org/1yn9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yn9 RCSB], [https://www.ebi.ac.uk/pdbsum/1yn9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yn9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PTP_NPVAC PTP_NPVAC] Also dephosphorylates seryl and threonyl residues.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yn/1yn9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yn9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Baculovirus RNA 5'-triphosphatase (BVP) exemplifies a family of RNA-specific cysteine phosphatases that includes the RNA triphosphatase domains of metazoan and plant mRNA capping enzymes. Here we report the crystal structure of BVP in a phosphate-bound state at 1.5 A resolution. BVP adopts the characteristic cysteine-phosphatase alpha/beta fold and binds two phosphate ions in the active site region, one of which is proposed to mimic the phosphate of the product complex after hydrolysis of the covalent phosphoenzyme intermediate. The crystal structure highlights the role of backbone amides and side chains of the P-loop motif (118)HCTHGXNRT(126) in binding the cleavable phosphate and stabilizing the transition state. Comparison of the BVP structure to the apoenzyme of mammalian RNA triphosphatase reveals a concerted movement of the Arg-125 side chain (to engage the phosphate directly) and closure of an associated surface loop over the phosphate in the active site. The structure highlights a direct catalytic role of Asn-124, which is the signature P-loop residue of the RNA triphosphatase family and a likely determinant of the specificity of BVP for hydrolysis of phosphoanhydride linkages.
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===Crystal structure of baculovirus RNA 5'-phosphatase complexed with phosphate===
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Crystal structure of baculovirus RNA triphosphatase complexed with phosphate.,Changela A, Martins A, Shuman S, Mondragon A J Biol Chem. 2005 May 6;280(18):17848-56. Epub 2005 Feb 15. PMID:15713658<ref>PMID:15713658</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15713658}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1yn9" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15713658 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15713658}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1YN9 is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Autographa_californica_nucleopolyhedrovirus Autographa californica nucleopolyhedrovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YN9 OCA].
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==Reference==
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<ref group="xtra">PMID:15713658</ref><references group="xtra"/>
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[[Category: Autographa californica nucleopolyhedrovirus]]
[[Category: Autographa californica nucleopolyhedrovirus]]
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[[Category: Polynucleotide 5'-phosphatase]]
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[[Category: Large Structures]]
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[[Category: Changela, A.]]
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[[Category: Changela A]]
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[[Category: Martins, A.]]
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[[Category: Martins A]]
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[[Category: Mondragon, A.]]
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[[Category: Mondragon A]]
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[[Category: Shuman, S.]]
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[[Category: Shuman S]]
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[[Category: Cysteine phosphatase]]
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[[Category: P-loop]]
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[[Category: Rna triphosphatase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 13:59:39 2009''
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Current revision

Crystal structure of baculovirus RNA 5'-phosphatase complexed with phosphate

PDB ID 1yn9

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