2fcd

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{{Seed}}
 
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[[Image:2fcd.png|left|200px]]
 
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==Solution structure of N-lobe Myosin Light Chain from Saccharomices cerevisiae==
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The line below this paragraph, containing "STRUCTURE_2fcd", creates the "Structure Box" on the page.
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<StructureSection load='2fcd' size='340' side='right'caption='[[2fcd]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2fcd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FCD FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fcd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fcd OCA], [https://pdbe.org/2fcd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fcd RCSB], [https://www.ebi.ac.uk/pdbsum/2fcd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fcd ProSAT]</span></td></tr>
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{{STRUCTURE_2fcd| PDB=2fcd | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MLC1_YEAST MLC1_YEAST] Essential light chain for the class II conventional myosin MYO1. Acts also as light chain for the class V unconventional myosin MYO2 and for IQG1. Involved in the assembly of the contractile actomyosin ring at the bud neck during cytokinesis by recruiting IQG1 to the bud neck. Also required for chitin and MYO2-dependent secretory vesicle deposition to the center of the bud neck for septum formation. May stabilize MYO2 by binding to its IQ domains. Its major function is probably not to regulate MYO1 activity, but rather to coordinate actin ring formation and targeted membrane deposition during cytokinesis via its interactions with MYO1, IQG1 and MYO2.<ref>PMID:9700160</ref> <ref>PMID:12456647</ref> <ref>PMID:15210731</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fc/2fcd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fcd ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Calmodulin, regulatory, and essential myosin light chain are evolutionary conserved proteins that, by binding to IQ motifs of target proteins, regulate essential intracellular processes among which are efficiency of secretory vesicles release at synapsis, intracellular signaling, and regulation of cell division. The yeast Saccharomyces cerevisiae calmodulin Cmd1 and the essential myosin light chain Mlc1p share the ability to interact with the class V myosin Myo2p and Myo4 and the class II myosin Myo1p. These myosins are required for vesicle, organelle, and mRNA transport, spindle orientation, and cytokinesis. We have used the budding yeast model system to study how calmodulin and essential myosin light chain selectively regulate class V myosin function. NMR structural analysis of uncomplexed Mlc1p and interaction studies with the first three IQ motifs of Myo2p show that the structural similarities between Mlc1p and the other members of the EF-hand superfamily of calmodulin-like proteins are mainly restricted to the C-lobe of these proteins. The N-lobe of Mlc1p presents a significantly compact and stable structure that is maintained both in the free and complexed states. The Mlc1p N-lobe interacts with the IQ motif in a manner that is regulated both by the IQ motifs sequence as well as by light chain structural features. These characteristic allows a distinctive interaction of Mlc1p with the first IQ motif of Myo2p when compared with calmodulin. This finding gives us a novel view of how calmodulin and essential light chain, through a differential binding to IQ1 of class V myosin motor, regulate this activity during vegetative growth and cytokinesis.
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===Solution structure of N-lobe Myosin Light Chain from Saccharomices cerevisiae===
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Structural basis for the interaction of the myosin light chain Mlc1p with the myosin V Myo2p IQ motifs.,Pennestri M, Melino S, Contessa GM, Casavola EC, Paci M, Ragnini-Wilson A, Cicero DO J Biol Chem. 2007 Jan 5;282(1):667-79. Epub 2006 Oct 29. PMID:17074768<ref>PMID:17074768</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2fcd" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_17074768}}, adds the Publication Abstract to the page
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*[[Myosin 3D Structures|Myosin 3D Structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 17074768 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17074768}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2FCD is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FCD OCA].
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==Reference==
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<ref group="xtra">PMID:17074768</ref><references group="xtra"/>
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Cicero, D O.]]
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[[Category: Cicero DO]]
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[[Category: Contessa, G M.]]
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[[Category: Contessa GM]]
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[[Category: Melino, S.]]
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[[Category: Melino S]]
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[[Category: Paci, M.]]
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[[Category: Paci M]]
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[[Category: Pennestri, M.]]
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[[Category: Pennestri M]]
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[[Category: Ragnini-Wilson, A.]]
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[[Category: Ragnini-Wilson A]]
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[[Category: Ef-hand protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 14:33:55 2009''
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Current revision

Solution structure of N-lobe Myosin Light Chain from Saccharomices cerevisiae

PDB ID 2fcd

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