2erh

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{{Seed}}
 
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[[Image:2erh.png|left|200px]]
 
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==Crystal Structure of the E7_G/Im7_G complex; a designed interface between the colicin E7 DNAse and the Im7 immunity protein==
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The line below this paragraph, containing "STRUCTURE_2erh", creates the "Structure Box" on the page.
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<StructureSection load='2erh' size='340' side='right'caption='[[2erh]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2erh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ERH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ERH FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2erh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2erh OCA], [https://pdbe.org/2erh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2erh RCSB], [https://www.ebi.ac.uk/pdbsum/2erh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2erh ProSAT]</span></td></tr>
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{{STRUCTURE_2erh| PDB=2erh | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IMM7_ECOLX IMM7_ECOLX] This protein is able to protect a cell, which harbors the plasmid ColE7 encoding colicin E7, against colicin E7, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. Dimeric ImmE7 may possess a RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream ceiE7 gene as well as degradation of the upstream ceaE7 mRNA.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/er/2erh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2erh ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The redesign of protein-protein interactions is a stringent test of our understanding of molecular recognition and specificity. Previously we engineered a modest specificity switch into the colicin E7 DNase-Im7 immunity protein complex by identifying mutations that are disruptive in the native complex, but can be compensated by mutations on the interacting partner. Here we extend the approach by systematically sampling alternate rigid body orientations to optimize the interactions in a binding mode specific manner. Using this protocol we designed a de novo hydrogen bond network at the DNase-immunity protein interface and confirmed the design with X-ray crystallographic analysis. Subsequent design of the second shell of interactions guided by insights from the crystal structure on tightly bound water molecules, conformational strain, and packing defects yielded new binding partners that exhibited specificities of at least 300-fold between the cognate and the non-cognate complexes. This multi-step approach should be applicable to the design of polar protein-protein interactions and contribute to the re-engineering of regulatory networks mediated by protein-protein interactions.
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===Crystal Structure of the E7_G/Im7_G complex; a designed interface between the colicin E7 DNAse and the Im7 immunity protein===
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Computational design of a new hydrogen bond network and at least a 300-fold specificity switch at a protein-protein interface.,Joachimiak LA, Kortemme T, Stoddard BL, Baker D J Mol Biol. 2006 Aug 4;361(1):195-208. Epub 2006 May 24. PMID:16831445<ref>PMID:16831445</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2erh" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16831445}}, adds the Publication Abstract to the page
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*[[Colicin 3D structures|Colicin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16831445 is the PubMed ID number.
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*[[Colicin immunity protein 3D structures|Colicin immunity protein 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_16831445}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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2ERH is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ERH OCA].
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==Reference==
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<ref group="xtra">PMID:16831445</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Baker, D.]]
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[[Category: Large Structures]]
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[[Category: Joachimiak, L A.]]
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[[Category: Baker D]]
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[[Category: Kortemme, T.]]
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[[Category: Joachimiak LA]]
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[[Category: Stoddard, B L.]]
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[[Category: Kortemme T]]
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[[Category: Computational design]]
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[[Category: Stoddard BL]]
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[[Category: Hydrogen bond network]]
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[[Category: Molecular recognition]]
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[[Category: Protein complex]]
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[[Category: Redesigned protein-protein interface]]
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[[Category: Specificity]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 14:40:17 2009''
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Current revision

Crystal Structure of the E7_G/Im7_G complex; a designed interface between the colicin E7 DNAse and the Im7 immunity protein

PDB ID 2erh

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