2axr

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{{Seed}}
 
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[[Image:2axr.png|left|200px]]
 
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==Crystal structure of glucooligosaccharide oxidase from Acremonium strictum: a novel flavinylation of 6-S-cysteinyl, 8alpha-N1-histidyl FAD==
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The line below this paragraph, containing "STRUCTURE_2axr", creates the "Structure Box" on the page.
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<StructureSection load='2axr' size='340' side='right'caption='[[2axr]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2axr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sarocladium_strictum Sarocladium strictum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AXR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AXR FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.98&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABL:(2R,3R,4R,5R)-4,5-DIHYDROXY-2-(HYDROXYMETHYL)-6-OXOPIPERIDIN-3-YL+BETA-D-GLUCOPYRANOSIDE'>ABL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_2axr| PDB=2axr | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2axr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2axr OCA], [https://pdbe.org/2axr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2axr RCSB], [https://www.ebi.ac.uk/pdbsum/2axr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2axr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GOOX_SARSR GOOX_SARSR] Catalyzes the selective oxidation of C1 hydroxyl moieties on mono- and disaccharides with concomitant reduction of molecular oxygen to hydrogen peroxide. This results in the formation of the corresponding lactones, which typically undergo spontaneous hydrolysis. Glucooligosaccharide oxidase is able to oxidize the monosaccharide D-glucose as well as the disaccharides maltose, cellobiose, and lactose. In addition, it shows high selectivity for cello- and maltooligosaccharides, indicating that glucooligosaccharide oxidase prefers oligosaccharides with a beta-D-glucosyl unit on the reducing end and additional sugar units linked by alpha- or beta-1,4 glucosidic bonds.<ref>PMID:16332885</ref> <ref>PMID:1764476</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ax/2axr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2axr ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Glucooligosaccharide oxidase from Acremonium strictum has been screened for potential applications in oligosaccharide acid production and alternative carbohydrate detection, because it catalyzes the oxidation of glucose, maltose, lactose, cellobiose and cello- and maltooligosaccharides. We report the crystal structures of the enzyme and of its complex with an inhibitor, 5-amino-5-deoxy- cellobiono-1,5-lactam at 1.55- and 1.98-A resolution, respectively. Unexpectedly, the protein structure demonstrates the first known double attachment flavinylation, 6-S-cysteinyl, 8alpha-N1-histidyl FAD. The FAD cofactor is cross-linked to the enzyme via the C(6) atom and the 8alpha-methyl group of the isoalloxazine ring with Cys(130) and His(70), respectively. This sugar oxidase possesses an open carbohydrate-binding groove, allowing the accommodation of higher oligosaccharides. The complex structure suggests that this enzyme may prefer a beta-d-glucosyl residue at the reducing end with the conserved Tyr(429) acting as a general base to abstract the OH(1) proton in concert with the H(1) hydride transfer to the flavin N(5). Finally, a detailed comparison illustrates the structural conservation as well as the divergence between this protein and its related flavoenzymes.
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===Crystal structure of glucooligosaccharide oxidase from Acremonium strictum: a novel flavinylation of 6-S-cysteinyl, 8alpha-N1-histidyl FAD===
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Crystal structure of glucooligosaccharide oxidase from Acremonium strictum: a novel flavinylation of 6-S-cysteinyl, 8alpha-N1-histidyl FAD.,Huang CH, Lai WL, Lee MH, Chen CJ, Vasella A, Tsai YC, Liaw SH J Biol Chem. 2005 Nov 18;280(46):38831-8. Epub 2005 Sep 9. PMID:16154992<ref>PMID:16154992</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16154992}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2axr" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16154992 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16154992}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2AXR is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Acremonium_strictum Acremonium strictum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AXR OCA].
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[[Category: Sarocladium strictum]]
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[[Category: Huang C-H]]
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==Reference==
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[[Category: Lai W-L]]
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<ref group="xtra">PMID:16154992</ref><references group="xtra"/>
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[[Category: Liaw S-H]]
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[[Category: Acremonium strictum]]
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[[Category: Tsai Y-C]]
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[[Category: Huang, C H.]]
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[[Category: Vasella A]]
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[[Category: Lai, W L.]]
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[[Category: Liaw, S H.]]
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[[Category: Tsai, Y C.]]
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[[Category: Vasella, A.]]
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[[Category: Alpha+beta]]
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[[Category: Covalent flavoenzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 14:55:06 2009''
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Current revision

Crystal structure of glucooligosaccharide oxidase from Acremonium strictum: a novel flavinylation of 6-S-cysteinyl, 8alpha-N1-histidyl FAD

PDB ID 2axr

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